Articles tagged "Endothelial cells"

2022 American Transplant Congress
Sting but Not Mtor Signaling Regulates Exogenous Mitochondrion-Induced Endothelial Cell Mitophagy
H. Xu, L. Wang, A. D. Kirk, H. Xu
Duke University School of Medicine, Durham, NC
*Purpose: We have previously demonstrated activation and increasing mitophagy of human endothelial cells (ECs) following exogenous mitochondrion-stimulation. The objectives of this study were to evaluate…
2022 American Transplant Congress
Bcl6b is a Putative Regulator of Endothelial Identity and Endmt
N. M. Valenzuela
Pathology and Laboratory Medicine, UCLA, Los Angeles, CA
*Purpose: Loss of endothelial cell (EC) identity is central to endothelial to mesenchymal transition (EndMT) implicated in neointimal hyperplasia in transplant vasculopathy. However, the molecular…
2022 American Transplant Congress
HLA-II Subclasses Behave Differently Between Themselves and Between Humans: Cues to Understand Alloreactivity
S. Beland, O. Desy, M. Marcoux, M. Thivierge, J. Desgagnes, S. A. De Serres
Transplantation Unit, Renal Division, University Health Center of Quebec, Faculty of Medicine, Universite Laval, Quebec, QC, Canada
*Purpose: De novo anti-HLA-II DSAs (dnDSA) are associated with antibody-mediated rejection. However, some patients with anti-HLA-II DSAs will not develop rejection, suggesting that the development…
2022 American Transplant Congress
HDAC Inhibitors Suppress Endothelial Inflammation but Alter Identity
N. M. Valenzuela
Pathology and Laboratory Medicine, UCLA, Los Angeles, CA
*Purpose: Histone modifications such as acetylation control gene expression. Histone deacetylases (HDACs) have been proposed as an attractive therapeutic approach to counteract detrimental gene expression,…
2022 American Transplant Congress
Functional Recellularized Patient Derived Endothelium; A Human Vascular Graft Approach
H. Tejeda Mora¹, J. Willemse², Y. den Hartog¹, I. Schurink², M. M. Verstegen², J. de Jonge², R. C. Minnee², M. W. van den Hoogen², C. C. Baan¹, L. J. van der Laan², M. J. Hoogduijn¹
¹Transplant Institute - Internal Medicine, Erasmus Medical Center, Rotterdam, Netherlands, ²Transplant Institute - Department of Surgery, Erasmus Medical Center, Rotterdam, Netherlands
*Purpose: In transplantation, the endothelial lining is the first barrier between the donor organ and recipient immune system. Damaged endothelium exposes extracellular matrix (ECM) molecules…
2022 American Transplant Congress
Stabilization of Cell-Cell Junctions Improves Outcomes in an Ex-Vivo Porcine Renal Ischemia-Reperfusion Injury Culture Model
L. Plumblee¹, K. Patel², L. Langerude³, Z. Tu³, D. Nord⁴, T. Beduschi³, S. Nadig⁵, C. Atkinson⁶
¹MUSC College of Medicine, Gainesville, FL, ²University of Virgina, Charlottesville, VA, ³University of Florida, Gaineville, FL, ⁴Division of Pulmonary, Critical Care, and Sleep Medicine, University of Florida, Gainesville, FL, ⁵Northwestern University, Charleston, SC, ⁶Pulmonary Medicine, University of Florida, Gainesville, FL
*Purpose: Renal transplant is an accepted therapy for end-stage renal failure. Chronic transplant dysfunction remains the leading cause of long-term graft loss and is, in…
2022 American Transplant Congress
Selective Vascular Decellularization and Endothelial Cells Replacement as a Strategy to Reduce Reperfusion Injury in Bioartificial Organs
G. Haeublein¹, G. Lombardi¹, F. Caro¹, R. Carla¹, D. Guerrieri¹, C. Incardona², D. Casadei³, E. Chuluyan¹
¹CEFYBO, Buenos Aires, Argentina, ²GADOR S.A., Buenos Aires, Argentina, ³Fundación Argentina de Trasplante, Tercer Milenio, Buenos Aires, Argentina
*Purpose: The number of kidney transplants performed each year cannot meet the increasing demand of these organs. Several strategies aim to increasing the offer of…
2022 American Transplant Congress
Enhanced Delivery of Nanomedicine to Human Kidney During Ex Vivo Perfusion
C. Albert¹, L. Bracaglia¹, A. Koide², J. R. DiRito¹, T. Lysyy¹, E. Christopher¹, J. Grundler¹, J. T. Langford¹, D. Haakinson¹, J. S. Pober¹, W. M. Saltzman¹, S. Koide², G. Tietjen¹
¹Yale University, new haven, CT, ²Perlmutter Cancer Center, New York University Langone Medical Center, New York, NY
*Purpose: An efficient delivery of therapeutic agents to graft endothelial cells (ECs) prior to transplantation could render organs more resistant to injury, improving clinical outcomes…
2021 American Transplant Congress
Single-Cell RNA-seq Identifies Intra-Graft Population Heterogeneity in Acute Heart Allograft Rejection in the Mouse
C. Wu¹, Y. Tang¹, X. Shi¹, X. He¹, X. Li²
¹The First Affiliated Hospital,Sun Yat-sen University, Guangzhou, China, ²Immunobiology & Transplant Sciences Houston Methodist Hospital, Houston, TX
*Purpose: Transplant rejection remains a major barrier to graft survival and involves a diversity of cell types. But the heterogeneity in a given cell type…
2021 American Transplant Congress
Extracellular Matrix Injury of Kidney Allografts in Antibody-mediated Rejection
S. Clotet Freixas¹, C. McEvoy¹, I. Batruch², C. Pastrello³, M. Kotlyar³, J. Van¹, M. Arambewela¹, A. Boshart¹, S. Farkona¹, Y. Niu³, Y. Li¹, O. Famure¹, A. Bozovic⁴, V. Kulasingam⁴, P. Chen¹, J. S. Kim¹, E. Chan⁵, S. Moshkelgosha¹, S. A. Rahman⁶, J. Das⁶, T. Martinu¹, S. Juvet¹, I. Jurisica³, A. Chruscinski¹, R. John¹, A. Konvalinka¹
¹Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada, ²Department of Laboratory Medicine and Pathobiology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada, ³Krembil Research Institute, University Health Network, Toronto, ON, Canada, ⁴Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada, ⁵Department of Medicine, Division of Nephrology, University Health Network, Toronto, ON, Canada, ⁶Center for Systems Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA
*Purpose: Antibody-mediated rejection (AMR) accounts for >50% of kidney graft losses. AMR is caused by donor-specific antibodies (DSA) against HLA and non-HLA antigens in glomeruli…