Articles tagged "Endothelial cells"

2022 American Transplant Congress
3D Co-Culture System with Porcine Aortic Endothelial Cell Line and Human or Non-Human Primate Cells for Application in the Study of Xeno-Immune Reaction
S. K. Yadav¹, Y. Lee², S. Hurh³, S. Kim², J. Yang⁴, J. C. Jeong²
¹Medicine, Seoul National University, Seoul, Korea, Republic of, ²Internal Medicine (Nephrology), Seoul National University Bundang Hospital, Seongnam, Korea, Republic of, ³Medicine, Korea University, Seoul, Korea, Republic of, ⁴Internal Medicine (Nephrology), Yonsei University, Seoul, Korea, Republic of
*Purpose: Neutrophil Extracellular Trap (NET) is a vigorous immune reaction of neutrophil. It plays role in xenotransplantation rejection. Functional assay of NET is important; however,…
2022 American Transplant Congress
MTOR Inhibition Acts Directly on Endothelial Cells to Induce Contact-Independent Immune-Modulation of Allo-Specific T Cell Activation
H. Xu, L. Wang, V. A. Bendersky, Q. Gao, Q. Li, A. D. Kirk, H. Xu
Duke University School of Medicine, Durham, NC
*Purpose: Endothelial cells (ECs) form a barrier between vascularized organ allografts and host immunity and are known to provoke alloimmune responses. The mTOR inhibitor rapamycin…
2022 American Transplant Congress
Engineered Adaptable Human Endothelial Cells Enable Durable Engraftment of Subcutaneously Transplanted Islets
R. Craig-Schapiro, G. Li, S. Rafii
Weill Cornell Medical College, New York, NY
*Purpose: The current clinical practice of islet transplantation into the portal vein is suboptimal, with reduced vascularization limiting successful engraftment and the inability for monitoring…
2022 American Transplant Congress
Pd-1 Licenses Activated Treg for Lymphatic Migration
W. Piao¹, L. Li¹, V. Saxena¹, K. Hippen², M. Shirkey¹, B. Blazar², I. Lape³, y. zhang³, J. Bromberg¹
¹Surgery, University of Maryland, Baltimore, MD, ²University of Minnesota, Minneapolis, MN, ³Harvard Medical School, Boston, MA
*Purpose: In addition to serving as fluid conduits, lymphatic vessels are critical portals for immune cell migration. Lymphatic endothelial cells (LECs) constitutively express high levels…
2022 American Transplant Congress
Treg Migration and Suppression Require Engagement with Lymphotoxin Beta Receptor on Lymphatic Endothelial Cells to Protect Islet Allografts
V. Saxena¹, W. Piao¹, L. Li¹, M. WillsonShirkey¹, R. Lakhan¹, S. Walden², K. L. Hippen², B. R. Blazar², J. S. Bromberg¹
¹University of Maryland School of Medicine, Baltimore, MD, ²University of Minnesota, Minneapolis, MN
*Purpose: Treg sequential migration from graft to draining lymph nodes (dLN) via afferent lymphatics is required for Treg suppressive function. Treg lymphotoxin alpha beta (LTαβ)…
2022 American Transplant Congress
Polarized Il-6 Secretion from Lymphatic Endothelial Cells Promotes Treg Conversion to Extregs
V. Saxena¹, W. Piao¹, L. Li¹, M. W. Shirkey¹, R. Lakhan¹, S. Walden², K. L. Hippen², B. Blazar², J. S. Bromberg¹
¹University of Maryland School of Medicine, Baltimore, MD, ²University of Minnesota, Minneapolis, MN
*Purpose: Regulatory T cell (Treg) suppressor function and allograft protection depend on maintenance of transcription factor Foxp3 expression and lymphatic migration. Lymphatic endothelial cell (LEC)…
2022 American Transplant Congress
Cd80 on Effector T Cell Binds Pd-l1 on Lymphatic Endothelial Cells to Fine Tune Migration
W. Piao¹, V. Saxena¹, L. Li¹, K. Hippen², Y. Zhang², B. Blazar², I. Lape³, L. Riella³, J. Bromberg¹
¹Surgery, University of Maryland, Baltimore, MD, ²University of Minnesota, Minneapolis, MN, ³Harvard Medical School, Boston, MA
*Purpose: Antigen presenting cell (APC) CD80 trans-binds CD28 and CTLA4 on T cells, boosting or inhibiting immunity, respectively. CD80 has another ligand, Programmed Death-1 Ligand…
2022 American Transplant Congress
Sting but Not Mtor Signaling Regulates Exogenous Mitochondrion-Induced Endothelial Cell Mitophagy
H. Xu, L. Wang, A. D. Kirk, H. Xu
Duke University School of Medicine, Durham, NC
*Purpose: We have previously demonstrated activation and increasing mitophagy of human endothelial cells (ECs) following exogenous mitochondrion-stimulation. The objectives of this study were to evaluate…
2021 American Transplant Congress
Resolution of Endothelial Inflammation is Delayed Following IFNγ, Which Provokes a Long-lasting Pro-adhesive Phenotype Dependent on JAK/STAT Signaling
N. M. Valenzuela
Pathology and Laboratory Medicine, UCLA, Los Angeles, CA
*Purpose: Although many cytokines are upregulated within allografts undergoing rejection, intragraft transcripts are dominated by IFN-response signatures. Resolution is a key phase of inflammation needed…
2021 American Transplant Congress
Endothelial Glycocalyx Shedding is Associated with Graft Quality During Ex Vivo Lung Perfusion
K. Noda, B. J. Philips, T. Harano, J. D. Luketich, P. G. Sanchez
Department of Cardiothoracic Surgery, University of Pittsburgh, Pittsburgh, PA
*Purpose: Endothelial glycocalyx is the surface protective layer of the endothelial cells and its damage is known to be strongly associated with organ dysfunction. However,…