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Articles tagged "Endothelial cells"

  • 2016 American Transplant Congress

    Recombinant TSGL-Ig Protects Liver Endothelial Cells Against Prolonged Cold Preservation Injury in Mouse Liver Transplantation.

    C. Zhang,1,2 H. Ji,1 Y. Liu,1,2 Y. Zhang,1,2 X.-D. Shen,1 F. Gao,1 R. Busuttil, G. Shaw, J. Kupiec-Weglinski.

    1Dumont-UCLA Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, David Geffen School of Medicine at University of California-Los Angeles, Los Angeles, CA; 2Department of Surgery, Division of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.

    Background: Liver endothelial cells (LEC) suffer from ROS-mediated stress in the pathogenesis of liver ischemia-reperfusion injury (IRI). We used a novel recombinant form of soluble…
  • 2016 American Transplant Congress

    Sphingosine-1 Phosphate Receptor 1 Agonist SEW2871 Ameliorates Hepatocellular Injury on Ischemic Reperfusion in Mice: A New Therapeutic Target.

    T. Ito, N. Kuriyama, A. Matsuda, H. Kato, Y. Iizawa, Y. Murata, A. Tanemura, Y. Azumi, M. Kishiwada, S. Mizuno, M. Usui, H. Sakurai, S. Isaji.

    Hepatobiliary Pancreatic and Transplant Surgery, Mie University Graduate School of Medicine, Tsu City, Mie, Japan.

    Introduction: We had reported that activated protein C (APC) administration had cytoprotective effects on hepatic ischemic reperfusion (IR) injury in rat and mice models. Recent…
  • 2016 American Transplant Congress

    Prolonged Cold Ischemia Time Negatively Impacts Graft and Recipient Survival in a Murine Orthotopic Hindlimb Transplant Model.

    N. Datta, S. Devaney, A. Allen, R. Busuttil, K. Azari, J. Kupiec-Weglinski.

    Department of Surgery, UCLA Medical Center, Los Angeles, CA.

    Purpose: The safe maximum cold preservation time remains unknown in Vascularized Composite Allotransplantation (VCA), yet is of critical clinical significance. VCAs with a large muscular…
  • 2016 American Transplant Congress

    Hypothermic Machine Perfusion Ameliorates Vasospasm and Edema of Podocytes and Renal Tubular Epithelial Cells Through Upregulating Expression of Krüppel-Like Factors 2.

    Z. Zhong,1 D. Ko,2 Y. Wang,1 Q. Ye.1

    1Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Wuhan, Hubei, China; 2Massachusetts General Hospital, Department of Urology, Harvard Medical School, Boston, MA.

    Protective mechanisms of hypothermic machine perfusion (MP) remain largely unknown. Vascular branches of the renal arteries of 6 kidneys in 320 donors were unexpectedly injured…
  • 2016 American Transplant Congress

    Detection of Endothelial Cell Specific Molecule-1 According to Allograft Status After Kidney Transplantation.

    J.-W. Seo, S.-Y. Kim, Y.-G. Kim, Y.-H. Jeong, Y.-H. Lee, J.-Y. Moon, T.-W. Lee, C.-G. Ihm, S.-H. Lee.

    Division of Nephrology, Department of Internal Medicine, College of Medicine, Kyung Hee University, Seoul, Republic of Korea.

    To investigate urine ESM-1(endothelial cell specific molecule-1) as well as plasma ESM-1 level from renal allograft recipients, we show whether there is the difference of…
  • 2016 American Transplant Congress

    Everolimus Abrogates C5a and TGFβ Mediated Perycite-to-Myofibroblast Transition (PMT) Inhibiting Vascular Rarefaction.

    G. Castellano, R. Franzin, A. Stasi, C. Divella, L. Gesualdo.

    Department of Emergency and Organ Transplantation, University of Bari, Bari, Italy.

    Microvascular pericytes have detrimental effects on tubulo-interstitial fibrosis via PMT. However, therapeutic strategies that might interfere with this process are currently lacking. We aimed to…
  • 2016 American Transplant Congress

    Human and Mouse Treg Use Lymphotoxin for Migration Across Lymphatic Endothelium.

    C. Brinkman,1 K. Hippen,2 B. Blazar,2 J. Bromberg.1

    1University of Maryland, Baltimore, MD; 2Univerity of Minnesota, Minneapolis, MN.

    Introduction: Lymphotoxin (LT) directs lymphoid organ structure, and LTβ receptor (LTβR) is involved in leukocyte migration into lymph nodes (LN) and thymocyte migration. We previously…
  • 2016 American Transplant Congress

    The 20S Proteasome, Active within Exosome-Like Vesicles Released by Apoptotic Cells Accelerates Rejection.

    M. Dieudé,1,4,5 C. Bell,2,5 J. Turgeon,1,5 D. Beillevaire,1,4,5 S. Qi,1,5 E. Boilard,3,5 P. Thibault,2,5 M.-J. Hébert.1,4,5

    1Research Centre Centre hospitalier de l'Université de Montréal (CRCHUM), Montréal, Canada; 2Institute for research in immunology and cancer (IRIC), Montreal, Canada; 3Centre de Recherche du CHU de Québec, Québec, Canada; 4Université de Montréal, Montréal, Canada; 5Canadian National Transplant Research Program, Edmonton, Canada.

    Apoptotic bodies (ApoBodies) and apoptotic exosome-like MV (ApoExo) released by apoptotic endothelial cells in vitro were purified by sequential centrifugation and analysed by electron microscopy…
  • 2016 American Transplant Congress

    Blocking MHC Class II on Human Endothelium Mitigates Acute Rejection of Vessel Allografts by Effector Memory T Cells.

    P. Abrahimi,1 L. Qin,2 W. Chang,1 A. Bothwell,1 G. Tellides,2 W. Saltzman,3 J. Pober.1

    1Immunobiology, Yale School of Medicine, New Haven, CT; 2Surgery, Yale School of Medicine, New Haven, CT; 3Biomedical Engineering, Yale University, New Haven, CT.

    Acute allograft rejection is mediated by alloreactive CD8+ T cells that differentiate into cytotoxic lymphocytes (CTL). In most rodent models, CTL differentiate from naïve CD8+…
  • 2016 American Transplant Congress

    Pathogenic Role of Anti-HLA Antibodies on Endothelial Progenitor Cell Dysfunction of Highly Sensitized Kidney Transplanted Patients.

    V. Cantaluppi,1 S. Dellepiane,2 D. Medica,2 G. Tognarelli,2 M. Quaglia,1 C. Musetti,1 A. Airoldi,1 L. Biancone.2

    1Nephrology, Dialysis and Kidney Transplantation Unit, University of Eastern Piedmont "A. Avogadro", Novara, Italy; 2Nephrology, Dialysis and Kidney Transplantation Unit, University of Torino, Torino, Italy.

    Anti-HLA antibodies (abs) bind to kidney graft endothelial cells inducing complement-mediated injury and humoral rejection. Endothelial Progenitor Cells (EPC) are bone marrow-derived precursors able to…
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