Articles tagged "Endothelial cells"

2018 American Transplant Congress
Double Deficiency of Gelatinase-A and Gelatinase-B Does Not Protect against Hepatic Ischemia Reperfusion Injury
S. Duarte, P. Matian, S. Ma, R. Busuttil, A. Coito.
Dumont-UCLA Transplant Center, Los Angeles.
Gelatinases, consisting of gelatinase A (MMP-2; 72 KDa) and gelatinase B (MMP-9; 92KDa), are a subgroup of matrix metalloproteinases (MMPs) with key roles in a…
2018 American Transplant Congress
Endothelial Progenitor Cell-Derived Extracellular Vesicles Inhibit Kidney Ischemia-Reperfusion Injury through the Transfer of Specific microRNA and mRNA Coding for the Complement Inhibitors CD55, CD59, Factor H and for the Transcription Factor NRF2: Relevance for Delayed Kidney Graft Function
V. Cantaluppi,¹ D. Medica,¹ S. Dellepiane,¹ G. Merlotti,¹ A. Airoldi,¹ M. Quaglia,¹ G. Castellano,² L. Gesualdo,² G. Camussi.³
¹Nephrology and Kidney Transplantation Unit, University of Piemonte Orientale (UPO), Novara, Italy; ²Nephrology, Dialysis and Kidney Transplantation Unit, University of Bari, Bari, Italy; ³Department of Medical Sciences, University of Torino, Torino, Italy.
Complement activation and oxidative cell damage are hallmarks of delayed kidney graft function (DGF) due to ischemia-reperfusion injury (IRI). Endothelial progenitor cells (EPC) protect from…
2018 American Transplant Congress
Mitochondrial Membrane Permeability Plays a Critical Role for Endothelial Cell Necroptosis and Cardiac Allograft Rejection
I. Gan, J. Jiang, D. Lian, X. Huang, W. Liu, A. Haig, A. Jevnikar, Z. Zhang.
Matthew Mailing Centre for Translational Transplant Studies, University of Western Ontario, London, ON, Canada; Multi Organ Transplant Program, London Health Sciences Centre, London, ON, Canada; Pathology and Laboratory Medicine, University of Western Ontario, London, ON, Canada; Medicine, University of Western Ontario, London, ON, Canada.
Background: Transplant injury is invariably associated with programmed cell death resulting in delayed graft function and organ rejection. We were the first to describe receptor-interacting…
2018 American Transplant Congress
Assessing the Impact of Flow in Hypothermic Machine Perfusion on Human Venous Endothelium Using a Novel Ex Vivo Model
Y. Marie,¹ W. Ries,² K. Patel,¹ C. Turnbull,² N. Jamil,⁴ H. Caldwell,³ R. Telfer,² D. Neil,⁵ J. Nath,¹ N. Inston.¹
¹Department of Renal Surgery, Queen Elizabeth Hospital, Birmingham, West Midlands, United Kingdom; ²College of Medical and Dental Sciences, University of Birmingham, Birmingham, West Midlands, United Kingdom; ³Division of Pathology Laboratories, Western General Hospital, Edinburgh, United Kingdom; ⁴Department of Cellular Pathology, Durham and Darlington NHS Foundation Trust, Newcastle, Newcastle Upon Tyne, United Kingdom; ⁵Department of Histopathology, University Hospitals Birmingham NHS Trust, Birmingham, West Midlands, United Kingdom.
BACKGROUNDHypothermic machine perfusion (HMP) involves dynamic flow of chilled preservation fluid through an allograft's vasculature. This study describes a simple, reproducible, human model that allows…
2018 American Transplant Congress
A Novel Endothelial Cell Based Complement Dependent Cytotoxicity Test in Kidney Transplantation
R. Lammerts,¹ J. van den Born,¹ W. Dam,¹ M. Seelen,¹ B. Hepkema,² B. Kroesen,² M. Daha,¹ R. Pol,³ S. Berger,¹ On behalf of the COMBAT Consortium.¹
¹Department of Nephrology, University Medical Centre Groningen, Groningen, Netherlands; ²Department of Immunology, University Medical Centre Groningen, Groningen, Netherlands; ³Department of Surgery, University Medical Centre Groningen, Groningen, Netherlands.
Introduction:Relevant alloantibodies in kidney transplantation comprise anti-HLA antibodies, blood group antibodies (ABO incompatible) and anti-endothelial cell antibodies (AECA) and can initiate antibody-mediated rejection (ABMR). ABMR…
2018 American Transplant Congress
Targeting LTβR Classical NFκB Signaling Facilitates Inflammation Resolution
W. Piao, Y. Xiong, L. Li, V. Saxena, J. Bromberg.
UMB, Baltimore.
Background:Contact hypersensitivity (CHS) is a reliable model to assess the immune cell-mediated cutaneous inflammatory response, which includes the three phases of priming/sensitization, elicitation, and resolution,…
2018 American Transplant Congress
Apoptotic Exosome-Like Vesicles Induce Endothelial Cell Plasticity
F. Migneault,^1,2,4 M. Dieudé,^1,2,4 J. Turgeon,^1,4 D. Beillevaire,^1,2,4 M-.P. Hardy,^2,3,4 C. Perreault,^2,3,4 M-.J. Hébert.^1,2,4
¹CRCHUM, Montreal, QC, Canada; ²Université de Montréal, Montreal, QC, Canada; ³IRIC, Montreal, QC, Canada; ⁴CNTRP, Montreal, QC, Canada.
Acute vascular rejection of renal allografts is associated with increased endothelial apoptosis which contributes to the development of transplant vasculopathy. We previously showed that apoptotic…
2018 American Transplant Congress
Involvement of PD-L1 Upregulation in Anti-A/B Antibody-Induced Graft Accommodation: Reduction of CD4 T Cell Allo-Response against Graft HLA Class II DR
K. Iwasaki,¹ Y. Miwa,¹ H. Hamana,³ H. Kishi,³ A. Muraguchi,³ K. Uchida,¹ T. Kobayashi.²
¹Kidney Disease and Transplant Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; ²Renal Transplant Surgery, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; ³Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
[Background] Antibodies against transplanted organs cause complement-independent graft injury, but there remain many unknown aspects of its mechanism, in particular its effect on allo-response to…
2018 American Transplant Congress
Endothelial Cell Heterogeneity Governs Basal and Inducible Inflammatory Responses Relevant to Allograft Injury
N. Valenzuela.
Pathology and Laboratory Medicine, UCLA, Los Angeles, CA.
PurposeTransplanted tissues are differentially susceptible to ischemia/reperfusion and rejection. Endothelial cells (EC) are active regulators of inflammation. But model EC may not adequately reflect the…
2018 American Transplant Congress
Targeting Lymphotoxin Signaling in Lymphatics Regulates Immune Sensitization, Elicitation, and Resolution
W. Piao, Y. Xiong, L. Li, V. Saxena, J. Bromberg.
UMB, MD.
Background:Contact hypersensitivity (CHS) is a T cell-mediated cutaneous inflammatory response which includes three phases of priming/sensitization, elicitation, and resolution,mirroring many aspects of allogeneic responses. The…