ATC Abstracts

American Transplant Congress abstracts

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Articles tagged "Endothelial cells"

  • 2019 American Transplant Congress

    Sphingosine 1-Phosphate Receptor 2 Regulates Migration through Endothelial Cell VCAM and Adherens Junctions

    Y. Xiong1, W. Piao1, C. Brinkman1, L. Li1, J. Kulinski2, A. Olivera2, A. Cartier3, T. Hla3, K. Hippen4, B. Blazar4, S. Schwab5

    1Surgery, UMB, Baltimore, MD, 2NIH, Bethesda, MD, 3Boston Children's hospital, Boston, MA, 4U.Minnesota, Minneapolis, MN, 5NYU, NY, NY

    *Purpose: Lymphatic endothelial cells (LEC) highly expres S1PR1 and S1PR2. The ligand S1P is produced by LEC and regulates T cell migration. The mechanisms of…
  • 2019 American Transplant Congress

    Use Of Perirenal Adipose Tissue As A Source Of Donor Cells Allowing Evaluation Of Endothelial Inflammation And Quality Of Expanded Criteria Kidneys

    R. Boissier1, L. Lyonnet2, P. Francois3, M. Meunier4, B. Gondran-Tellier5, T. Legris6, J. Magalon5, C. Picard7, F. Dignat George5, E. Lechevallier8, G. Karsenty8, F. Sabatier9, P. Paul1

    1INSERM 1263, Centre de recherche en cardiovasculaire et nutrition, INSERM, Aix Marseille Univ, Assistance Publique Hopitaux Marseille, Marseille, France, 2Vascular Biology and Hematology department, Assistance publique Hopitaux Marseille (AP-HM), Marseille, France, 3INSERM 1263, Centre de recherche en cardiovasculaire et nutrition, INSERM, Aix Marseille Univ., Marseille, France, 4Nephrology Dialysis and Renal Transplantation Center, AP-HM, Marseille, France, 5INSERM 1263, C2VN, INSERM, Aix Marseille Univ., Marseille, France, 6Nephrology Dialysis and Renal Transplantation Center, AP-HM, Marseille, France, 7Etablissement Français du Sang, Alpes Méditerranée, Marseille, France, 8Urology and Transplantation Unit , AP-HM, Marseille, France, 9INSERM 1263, Centre de recherche en cardiovasculaire et nutrition, INSERM, Aix Marseille Univ, Marseille, France

    *Purpose: Use of expanded-criteria donor (ECD) kidneys is a perspective to overcome organ shortage. Age and donor-related co-morbidy factors are known to affect graft function…
  • 2019 American Transplant Congress

    Inhibition of mTORC2 Causes Hyper Phosphorylation of ERK Induced by Co-Ligation of HLA-I and II Antibody

    Y. Jin1, J. Sinnet-Smith2, E. Rozengurt2, E. F. Reed1

    1Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, CA, 2Medicine, University of California Los Angeles, Los Angeles, CA

    *Purpose: Transplant recipients developing donor specific antibodies (DSA) are at risk for antibody-mediated rejection (AMR). It has been reported that as many as 30% of…
  • 2019 American Transplant Congress

    Stabilization of Cell Junctions Ameliorates the Effects of Brain Death Induced Acute Lung Injury Post-Transplantation

    D. P. Allen1, Z. Tu1, C. Wallace1, C. Li1, J. Kilkenny1, K. J. Patel1, D. Nord2, S. N. Nadig1, C. Atkinson2

    1Surgery, Medical University of South Carolina, CHARLESTON, SC, 2Microbiology & Immunology, Medical University of South Carolina, CHARLESTON, SC

    *Purpose: Donor Brain death (BD) induces acute lung injury (BD-ALI), characterized by the breakdown of endo/epithelial barrier functions. These injuries are further exacerbated by ischemia…
  • 2018 American Transplant Congress

    Assessing the Impact of Flow in Hypothermic Machine Perfusion on Human Venous Endothelium Using a Novel Ex Vivo Model

    Y. Marie,1 W. Ries,2 K. Patel,1 C. Turnbull,2 N. Jamil,4 H. Caldwell,3 R. Telfer,2 D. Neil,5 J. Nath,1 N. Inston.1

    1Department of Renal Surgery, Queen Elizabeth Hospital, Birmingham, West Midlands, United Kingdom; 2College of Medical and Dental Sciences, University of Birmingham, Birmingham, West Midlands, United Kingdom; 3Division of Pathology Laboratories, Western General Hospital, Edinburgh, United Kingdom; 4Department of Cellular Pathology, Durham and Darlington NHS Foundation Trust, Newcastle, Newcastle Upon Tyne, United Kingdom; 5Department of Histopathology, University Hospitals Birmingham NHS Trust, Birmingham, West Midlands, United Kingdom.

    BACKGROUNDHypothermic machine perfusion (HMP) involves dynamic flow of chilled preservation fluid through an allograft's vasculature. This study describes a simple, reproducible, human model that allows…
  • 2018 American Transplant Congress

    A Novel Endothelial Cell Based Complement Dependent Cytotoxicity Test in Kidney Transplantation

    R. Lammerts,1 J. van den Born,1 W. Dam,1 M. Seelen,1 B. Hepkema,2 B. Kroesen,2 M. Daha,1 R. Pol,3 S. Berger,1 On behalf of the COMBAT Consortium.1

    1Department of Nephrology, University Medical Centre Groningen, Groningen, Netherlands; 2Department of Immunology, University Medical Centre Groningen, Groningen, Netherlands; 3Department of Surgery, University Medical Centre Groningen, Groningen, Netherlands.

    Introduction:Relevant alloantibodies in kidney transplantation comprise anti-HLA antibodies, blood group antibodies (ABO incompatible) and anti-endothelial cell antibodies (AECA) and can initiate antibody-mediated rejection (ABMR). ABMR…
  • 2018 American Transplant Congress

    Targeting LTβR Classical NFκB Signaling Facilitates Inflammation Resolution

    W. Piao, Y. Xiong, L. Li, V. Saxena, J. Bromberg.

    UMB, Baltimore.

    Background:Contact hypersensitivity (CHS) is a reliable model to assess the immune cell-mediated cutaneous inflammatory response, which includes the three phases of priming/sensitization, elicitation, and resolution,…
  • 2018 American Transplant Congress

    Apoptotic Exosome-Like Vesicles Induce Endothelial Cell Plasticity

    F. Migneault,1,2,4 M. Dieudé,1,2,4 J. Turgeon,1,4 D. Beillevaire,1,2,4 M-.P. Hardy,2,3,4 C. Perreault,2,3,4 M-.J. Hébert.1,2,4

    1CRCHUM, Montreal, QC, Canada; 2Université de Montréal, Montreal, QC, Canada; 3IRIC, Montreal, QC, Canada; 4CNTRP, Montreal, QC, Canada.

    Acute vascular rejection of renal allografts is associated with increased endothelial apoptosis which contributes to the development of transplant vasculopathy. We previously showed that apoptotic…
  • 2018 American Transplant Congress

    Involvement of PD-L1 Upregulation in Anti-A/B Antibody-Induced Graft Accommodation: Reduction of CD4 T Cell Allo-Response against Graft HLA Class II DR

    K. Iwasaki,1 Y. Miwa,1 H. Hamana,3 H. Kishi,3 A. Muraguchi,3 K. Uchida,1 T. Kobayashi.2

    1Kidney Disease and Transplant Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; 2Renal Transplant Surgery, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; 3Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.

    [Background] Antibodies against transplanted organs cause complement-independent graft injury, but there remain many unknown aspects of its mechanism, in particular its effect on allo-response to…
  • 2018 American Transplant Congress

    Endothelial Cell Heterogeneity Governs Basal and Inducible Inflammatory Responses Relevant to Allograft Injury

    N. Valenzuela.

    Pathology and Laboratory Medicine, UCLA, Los Angeles, CA.

    PurposeTransplanted tissues are differentially susceptible to ischemia/reperfusion and rejection. Endothelial cells (EC) are active regulators of inflammation. But model EC may not adequately reflect the…
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