2019 American Transplant Congress
Ligation Of Hla Class Il Molecules Disrupts Endothelial Barrier Through Src-dependent Ve-cadherin Destabilization
*Purpose: Microvascular inflammation, vascular permeability, and accumulation of intravascular mononuclear cells are characteristics of acute and chronic antibody-mediated rejection. Vascular endothelial cadherin (VE-Cadherin) is a…2019 American Transplant Congress
Single Cell RNA-seq Analysis of Antibody-Mediated Rejection Reveals Endothelial – Pericyte NOTCH Signaling
Washington University School of Medicine, Saint Louis, MO
*Purpose: To precisely define endothelial cell responses in AMR we performed comprehensive single cell RNA-seq on a human kidney biopsy with AMR.*Methods: Single cell libraries…2019 American Transplant Congress
A Novel Ex Vivo Normothermic Perfusion Model of Human Antibody Mediated Rejection
*Purpose: Currently, no human experimental model of antibody-mediated rejection (AMR) exists, making the assessment of therapeutic interventions difficult hence the aim of our work is…2019 American Transplant Congress
Differential Regulation of Phosphokinases by Anti-HLA Class I and 11 Antibodies
*Purpose: Anti-HLA antibodies, both pre-formed and de novo are associated with worse graft survival. Glomerular endothelial cells are the targets for antibody mediated rejection. We…2019 American Transplant Congress
Clazakizumab Acts on Endothelial Cells to Limit Antibody Mediated Damage
*Purpose: Human microvascular endothelial cell expression of HLA class II antigens is strongly increased, both in vitro and in vivo, under inflammatory conditions. HLA class…2018 American Transplant Congress
Dual Inhibition of PI3K and mTORC2 Causes ERK Hyperactivation Induced by HLA-II Antibody in Human Endothelium
Pathology and Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA.
Background: Endothelial cells (EC) line the inner surface of blood vessels and serve as the interface between recipient circulating blood and the donor allograft. Transplant…2018 American Transplant Congress
Circulating Wnt Pathway Factors Are Non-Invasive Biomarkers of Transplant Vascular Injury
Purpose: Transplant vascular injury (TVI) is a major cause of renal allograft failure and is resistant to treatment. Non-invasive pathogenic biomarkers of TVI would provide…2018 American Transplant Congress
Ex Vivo Lung Perfusion Using the Portable OCS Maintains Endothelial Integrity in the Context of Reduced Severe PGD Rates
PurposeThe INSPIRE trial revealed significant reduction of PGD grade 3, i.e. less ischemia reperfusion injury (IRI) using the Organ Care System (OCS) compared to controls…2018 American Transplant Congress
An Increase in Spot Urine Protein Excretion during the First Year after Kidney Transplantation Associates with Subclinical De-Novo Donor-Specific Antibodies and Microvascular Inflammation at 1-Year Surveillance Biopsies
Introduction. We investigated whether an increase in spot urine protein excretion during the first year after kidney transplantation associates with subclinical de-novo donor-specific antibodies (DSA)…2018 American Transplant Congress
Assessing the Impact of Flow in Hypothermic Machine Perfusion on Human Venous Endothelium Using a Novel Ex Vivo Model
BACKGROUNDHypothermic machine perfusion (HMP) involves dynamic flow of chilled preservation fluid through an allograft's vasculature. This study describes a simple, reproducible, human model that allows…
American Transplant Congress abstracts