2019 American Transplant Congress
Novel Activation Responses by Endothelial Cells within the Pro-Tolerogenic Intragraft Microenvironment
*Purpose: Current paradigms indicate that endothelial cells (EC) within allografts participate in the initiation and progression of allograft rejection. However, little is known about their…2019 American Transplant Congress
Ligation Of Hla Class Il Molecules Disrupts Endothelial Barrier Through Src-dependent Ve-cadherin Destabilization
*Purpose: Microvascular inflammation, vascular permeability, and accumulation of intravascular mononuclear cells are characteristics of acute and chronic antibody-mediated rejection. Vascular endothelial cadherin (VE-Cadherin) is a…2019 American Transplant Congress
Single Cell RNA-seq Analysis of Antibody-Mediated Rejection Reveals Endothelial – Pericyte NOTCH Signaling
Washington University School of Medicine, Saint Louis, MO
*Purpose: To precisely define endothelial cell responses in AMR we performed comprehensive single cell RNA-seq on a human kidney biopsy with AMR.*Methods: Single cell libraries…2019 American Transplant Congress
A Novel Ex Vivo Normothermic Perfusion Model of Human Antibody Mediated Rejection
*Purpose: Currently, no human experimental model of antibody-mediated rejection (AMR) exists, making the assessment of therapeutic interventions difficult hence the aim of our work is…2019 American Transplant Congress
Differential Regulation of Phosphokinases by Anti-HLA Class I and 11 Antibodies
*Purpose: Anti-HLA antibodies, both pre-formed and de novo are associated with worse graft survival. Glomerular endothelial cells are the targets for antibody mediated rejection. We…2018 American Transplant Congress
Anti-Angiotensin II Type 1-Receptor Antibodies (AT1R-Ab) Induce a Specific Phenotype of Rejection Distinct from HLA Antibody-Mediated Rejection
AT1R-Ab have been associated with kidney allograft rejection; however, their ability to induce a specific rejection phenotype, independent of HLA-DSAs, has not been defined.In a…2018 American Transplant Congress
The Utility of a CD31/CD68 Dual Immunohistochemical Stain to Improve Accurate Diagnosis of Antibody Mediated Rejection in Cardiac Transplantation
Thoracic Pathology, University of Texas Southwestern Medical Center, Dallas, TX.
Antibody mediated rejection (AMR) occurs in 10%-20% of cardiac transplant patients and is associated with increased mortality. The endomyocardial biopsy, used to identify microvascular injury…2018 American Transplant Congress
Endothelial Cell Heterogeneity Governs Basal and Inducible Inflammatory Responses Relevant to Allograft Injury
Pathology and Laboratory Medicine, UCLA, Los Angeles, CA.
PurposeTransplanted tissues are differentially susceptible to ischemia/reperfusion and rejection. Endothelial cells (EC) are active regulators of inflammation. But model EC may not adequately reflect the…2018 American Transplant Congress
Dual Inhibition of PI3K and mTORC2 Causes ERK Hyperactivation Induced by HLA-II Antibody in Human Endothelium
Pathology and Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA.
Background: Endothelial cells (EC) line the inner surface of blood vessels and serve as the interface between recipient circulating blood and the donor allograft. Transplant…2018 American Transplant Congress
Circulating Wnt Pathway Factors Are Non-Invasive Biomarkers of Transplant Vascular Injury
Purpose: Transplant vascular injury (TVI) is a major cause of renal allograft failure and is resistant to treatment. Non-invasive pathogenic biomarkers of TVI would provide…
American Transplant Congress abstracts