Articles tagged "Biopsy"

2022 American Transplant Congress
Using a Unique Repository of Pancreas Transplant Biopsies to Validate the Tissue Common Response Module (tCRM) Score as a Tool to Assess the Severity of Pancreas Transplant Rejection
Y. Kelly¹, A. Zarinsefat¹, R. Meier², M. Sarwal¹, P. Stock¹, Z. Laszik¹, T. Sigdel¹
¹University of California, San Francisco, San Francisco, CA, ²University of Maryland Medical Center, Baltimore, MD
*Purpose: Analysis of transcriptional data from kidney, heart, liver, and lung transplant rejection led to development of the tissue Common Response Module (tCRM) score based…
2022 American Transplant Congress
The Prediction of Renal Allograft Rejection by Time-Dependent Two-Signature Predictors
M. Liao¹, Q. Fu², L. Wei³, H. Yang³, G. Zhao³, S. Deng³, J. F. Markmann²
¹School of Medicine, University of Electronic Science and Technology of China, Chengdu, China, ²Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ³University of Electronic Science and Technology of China, Chengdu, China
*Purpose: The study aims to develop RNA signature predictors using preemptive biopsy transcriptome data to predict future allograft rejection and reveal the predictors' time-dependent features.*Methods:…
2022 American Transplant Congress
Rapid Formalin-Fixed Deceased Donor Kidney Biopsy Protocol – A Proof Of Concept
M. R. Marvin¹, G. Chen², A. Kotru¹, C. Koo¹, P. Sanghi³, M. Moritz⁴, J. Clarke⁴, R. Hasz⁴
¹Transplant, Geisinger Medical Center, Danville, PA, ²Pathology, Geisinger Medical Center, Danville, PA, ³Transplant Nephrology, Geisinger Medical Center, Danville, PA, ⁴Gift of Life Donor Program, Philadelphia, PA
*Purpose: Reducing kidney discard is a major priority for the transplant community. While frozen section biopsies are the current standard, they are fraught with technical…
2022 American Transplant Congress
Temporal Changes in the Peripheral Immune Profile of Acute Rejection Highlight New Mechanisms of Alloimmunity and Potential Non-Invasive Biomarkers of Rejection in Pediatric Liver Transplantation
D. N. Sarode¹, B. Rocque¹, A. Barbetta¹, P. Singh¹, C. Goldbeck¹, C. Weaver², O. Akbari¹, R. Kohli², J. Emamaullee¹
¹University of Southern California Keck School of Medicine, Los Angeles, CA, ²Children's Hospital Los Angeles, Los Angeles, CA
*Purpose: Acute rejection (AR) is common in pediatric liver transplantation (LT) and can impact late graft loss. Immune subpopulations mediating AR in LT have not…
2022 American Transplant Congress
Novel Avenue of Allograft Monitoring: Direct Measurement of Donor-Derived Extracellular Vesicles in Human Plasma
W. W. Woud, D. A. Hesselink, M. J. Hoogduijn, C. C. Baan, K. Boer
Internal Medicine, Erasmus MC Transplant Institute, Rotterdam, Netherlands
*Purpose: Extracellular Vesicles (EV) - regarded as “snapshots” of their cell of origin - represent promising liquid biomarkers to monitor allograft function post transplantation. Recently,…
2022 American Transplant Congress
Deep Learning Identifies Arterial Intimal Fibrosis in Kidney Transplant Biopsies
K. Liu¹, Z. Yi², S. Ward³, F. Salem³, W. Zhang²
¹Nephrology, Icahn School of Medicine at Mount Sinai, New York, NY, ²Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, ³Molecular and Cell Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY
*Purpose: Arterial intimal fibrosis (AIF) is the excessive fibrotic tissue formation in the intima of arteries indicative of poor graft function after kidney transplant. Visual…
2022 American Transplant Congress
Role of Circulating Tumour Cells in the Management of the Liver Transplant Patient with Hepatocellular Carcinoma
F. Villalba¹, F. Alconchel², L. Sáenz³, M. Sánchez-Lorencio¹, D. Ferreras², P. Cascales², F. Sánchez-Bueno², R. Robles², P. Ramírez²
¹IMIB-Virgen de la Arrixaca, Murcia, Spain, ²Surgery and Organ Transplantation, Virgen de la Arrixaca University Hospital (IMIB-Virgen de la Arrixaca), Murcia, Spain, ³Rafael Méndez Hospital, Lorca, Spain
*Purpose: For hepatocellular carcinoma (HCC), liver transplantation (LT) is considered a curative treatment, however, more than 10% of transplant recipients have recurrences within the first…
2022 American Transplant Congress
Deep Phenotyping Methods Highlight Recipient-Derived CD16+ NK and CD16+ Monocyte Infiltration During Kidney Transplant Rejection
B. Lamarthée¹, J. Callemeyn¹, P. Koshy², T. Debyser³, E. Van Loon¹, A. Franken⁴, T. Van Brussel⁴, I. Arijs⁴, D. Lambrechts⁴, A. Senev¹, T. Vaulet⁵, C. Tinel¹, M. Naesens¹
¹Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium, ²Department of Morphology and Molecular Pathology, University Hospitals Leuven, Leuven, Belgium, ³Faculty of Medicine, KU Leuven, Leuven, Belgium, ⁴Laboratory for Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium, ⁵ESAT Stadius Center for Dynamical Systems, Signal Processing and Data Analytics, KU Leuven, Leuven, Belgium
*Purpose: How the immune system participates to kidney transplant rejection has not been fully elucidated. Single-cell (sc) genomics techniques are revolutionizing our ability to characterize…
2022 American Transplant Congress
Spatially Resolved Molecular Profiling of Endothelial Cells in Antibody-Mediated Kidney Transplant Rejection
M. Melendez-Ferro, D. R. Kelly, M. E. Seifert
UAB School of Medicine, Birmingham, AL
*Purpose: Endothelial cells are key targets of alloimmune injury in antibody-mediated rejection (ABMR), but are challenging to study in situ using high-throughput methods. We aimed…
2022 American Transplant Congress
Can dd-cf DNA Eliminate Unnecessary Biopsies? The Answer is Yes!
K. Andreoni, Y. Chalicheemala, M. Debroy, M. Maklad, K. Zhao, M. Aeder, A. Jittirat, D. Ecabert, A. Bixby, C. Burrelli, K. Chavin
University Hospitals Cleveland Medical Center, Cleveland, OH
*Purpose: Historically our center has been one that routinely performs biopsies. We perform them pre-perfusion, for slowly recovering allografts between day 10-14, and routinely at…