Articles tagged "Bioengineering"

2019 American Transplant Congress
Ex Vivo Assessment of Marginal Human Kidneys Using Computed Tomography
M. K. Harris¹, J. R. DiRito², H. Liu³, N. Boutagy³, C. Liu⁴, S. Hosgood², D. Mulligan¹, M. L. Nicholson², A. Sinusas³, D. Haakinson¹, G. T. Tietjen¹
¹Surgery, Yale School of Medicine, New Haven, CT, ²Surgery, University of Cambridge, Cambridge, United Kingdom, ³Yale Translational Research Imaging Center, New Haven, CT, ⁴Radiology, Yale School of Medicine, New Haven, CT
*Purpose: Thousands of ‘marginal’ organs are discarded each year despite a severe organ shortage. This has motivated development of new technologies, such as ex vivo…
2019 American Transplant Congress
MHC Molecule-Ablated Human Endothelial Cells Form Alloimmune-Evasive Microvessels
J. Merola¹, M. Reschke², L. Qin¹, S. Spindler¹, R. W. Pierce³, T. D. Manes⁴, G. Li¹, L. Bracaglia², N. Kirkiles-Smith⁴, T. Baltazar⁴, W. Saltzman², G. T. Tietjen¹, G. Tellides¹, J. S. Pober⁴
¹Surgery, Yale School of Medicine, New Haven, CT, ²Biomedical Engineering, Yale School of Engineering and Applied Science, New Haven, CT, ³Pediatrics, Yale School of Medicine, New Haven, CT, ⁴Immunobiology, Yale School of Medicine, New Haven, CT
*Purpose: “Off the shelf” tissue-engineered organs will require use of allogeneic cell sources. Allogeneic human endothelial cells (ECs), necessary for organ perfusion, can initiate graft…
2019 American Transplant Congress
Organ Transportation by Drone: Surgeon Perspectives
T. Talaie¹, R. Barth², J. R. Scalea¹
¹University of Maryland, Baltimore, MD, ²Surgery, University of Maryland, Baltimore, MD
*Purpose: We recently reported on the first transport of a human kidney using a drone. Ahead of large scale adoption of such a technology, understanding…
2019 American Transplant Congress
Hepatic Stellate Cells and Liver Derived Endothelial Cells Improve Scaffold-Free 3D-Bioprintied Liver Model
A. M. Chen¹, W. Zhang¹, C. J. Koehler¹, K. Saglam¹, A. Isidan¹, J. R. Walsh¹, R. A. Naqvi¹, E. Gramelspacher¹, L. J. Smith², P. Li¹, B. Ekser¹
¹Transplant Division, Department of Surgery, Indiana University, Indianapolis, IN, ²3D-Bioprinting Core, Indiana University School of Medicine, Indianapolis, IN
*Purpose: Hepatic stellate cells (HSC) comprise about 2-8% of liver cells and are vital to hepatocellular function. Scaffold-free 3D-bioprinting (SF3DBP) allows creation of realistic tissue…
2019 American Transplant Congress
Evaluating Liver Regeneration during Normothermic Machine Perfusion in Hepatectomized Rat Liver Model
M. Aburawi¹, S. Mert¹, E. Hafiz², P. Mahboub¹, H. Yeh¹, K. Uygun¹, J. Markmann¹
¹Surgery, Massachusetts General Hospital, Boston, MA, ²Pathology and Electron Microscopy, Theodor Bilharz Research Institute, Cairo, Egypt
*Purpose: Realizing the liver’s unique ability to regenerate, this ongoing study examines Normothermic Machine Perfusion (NMP) as a promising platform for evaluating & potentially simulating…
2019 American Transplant Congress
Kidney Regeneration with Biomimetic Vascular Scaffolds Based on Vascular Corrosion Casts
S. Min¹, H. Mo¹, A. Han¹, J. Ha¹, J. Yoo²
¹Seoul National University Hospital, Seoul, Korea, Republic of, ²Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC
*Purpose: We have developed a biomimetic renal vascular scaffold based on a vascular corrosion casting technique. In this study, we evaluated the feasibility of using…
2019 American Transplant Congress
Tolerogenic Nanoparticles Targeting the Aryl Hydrocarbon Receptor
K. Janek¹, J. Fechner¹, S. Gong², Y. Wang², J. Mezrich¹
¹Department of Surgery, Division of Transplantation, University of Wisconsin Hospital and Clinics, Madison, WI, ²Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI
*Purpose: The aryl hydrocarbon receptor (AHR) has been shown to have a central role in the ability of dendritic cells (DCs) to direct differentiation of…
2018 American Transplant Congress
Activity Nanosensors for Early and Noninvasive Detection of Acute Organ Transplant Rejection
Q. Mac,¹ D. Mathews,² J. Kahla,¹ C. Stoffers,¹ O. Delmas,¹ B. Holt,¹ A. Adams,² G. Kwong.¹
¹Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA; ²Department of Surgery, Emory University School of Medicine, Atlanta, GA.
Purpose: We engineered activity nanosensors that sense anti-allograft T cell activity in vivo to noninvasively detect the onset of acute cellular rejection from urine.Methods: Granzyme…
2018 American Transplant Congress
Engineering Regulatory T Cells with Interleukin (IL-)2 Nanogel for Improved Alloimmune Suppression
S. Eskandari,¹ M. Bandeira Melo,² J. Assaker,¹ A. Mansouri,¹ S. Cai,¹ B. Al Dulaijan,¹ M. Mohamed,¹ D. Irvine,² J. Azzi.¹
¹Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School, Boston, MA; ²Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA.
Clinical trials of adoptively transferred regulatory T cells (TRegs) have been hindered by the short lifespan of these transferred TRegs. Systemic injection of low-dose interleukin…
2018 American Transplant Congress
Sensing T Cell Killing Activity with Granzyme B Activity Probes
Q. Mac,¹ D. Mathews,² J. Kahla,¹ C. Stoffers,¹ O. Delmas,¹ B. Holt,¹ A. Adams,² G. Kwong.¹
¹Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA; ²Department of Surgery, Emory University School of Medicine, Atlanta, GA.
Purpose: We developed activity-based probes that sense Granzyme B (GzmB) activity during alloreactive T cell killing of donor cellsMethods: FITC-labelled GzmB substrate (IEFDSG) was synthesized…