Articles tagged "B cells"

2020 American Transplant Congress
Dissecting Humoral Alloimmune Sensitization: A Dynamic Interplay of Different Cellular Compartments
A. Torija, A. Favà, E. Crespo, M. Jarque, M. Meneghini, N. Bolaños, J. Grinyo, O. Bestard
Kidney transplantation Unit, Bellvitge University Hospital- IDIBELL, L'Hospitalet de Llobregat. Barcelona, Spain
*Purpose: Humoral alloimmune memory is the main barrier for successful transplantation and is generated by a complex and compartmentalized B-cell immune response. Understanding the role…
2020 American Transplant Congress
Activated CD27⁺CD21^– Subset Dominate the Memory B Cell Response During Antibody-Mediated Rejection of Kidney Allografts
K. Louis¹, E. Bailly¹, C. Macedo¹, M. Marrari¹, M. Yamada¹, P. Randhawa¹, A. Zeevi¹, C. Lefaucheur², D. Metes¹
¹Surgery, University of Pittsburgh, Pittsburgh, PA, ²Inserm UMR 976, Paris, France
*Purpose: The role of alloreactive memory B (mB) cells in the pathogenesis of antibody-mediated rejection (ABMR) is increasingly recognized. Yet, a comprehensive characterization of mB…
2020 American Transplant Congress
Thymic B Cells: A Means to Control Regulatory Tolerance
C. LeGuern¹, S. Germana¹, N. Feeney¹, Z. Wang², N. Tanimine¹, C. Huang³, C. Rickert¹, J. Markmann¹
¹Surgery, Center for Transplantation Sciences, Massachusetts General Hospital, Charlestown, MA, ²University of Colorado Denver, Aurora, CO, ³Surgery, University of Colorado Denver, Aurora, CO
*Purpose: Therapies involving CD4 regulatory T cells (Tregs) are promising approaches to mitigate graft rejection and autoimmunity. Understanding of the activation mechanisms that initiate Treg…
2020 American Transplant Congress
Characterization of Non HLA Antibodies in Kidney Transplant Recipients
S. Rodriguez Ramirez¹, S. Clotet-Freixas¹, C. Mcevoy¹, M. Kotlyar², I. Jurisica², A. Chruscinski¹, A. Konvalinka³
¹Kidney Transplant, Toronto General Hospital Research Institute, Multi-Organ Transplant Program, University Health Network, Toronto, ON, Canada, ²Kidney Transplant, Krembil Research Institute, University Health Network, Toronto, ON, Canada, ³Kidney Transplant, Department of Medicine, Division of Nephrology, University Health Network, Toronto, ON, Canada
*Purpose: Antibody mediated rejection (AMR) causes more than 60 percent of late graft failures in kidney transplants (KT). Although antiHLA donor specific antibodies are the…
2020 American Transplant Congress
Allograft Repopulating Recipient B Cells after Human Intestinal Transplantation and Their Association with Donor Specific Antibody Secretion and Rejection
E. Waffarn¹, J. Fu¹, K. Frangaj¹, W. Meng², A. M. Rosenfeld², M. Li³, K. Rodgers¹, R. Vasilescu³, E. T. Luning Prak², A. Griesemer¹, T. Kato⁴, M. Martinez⁵, M. Sykes¹
¹Columbia Center for Translational Immunology, Department of Medicine, Columbia University, New York, NY, ²Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, ³Department of Pathology, Columbia University, New York, NY, ⁴Department of Surgery, Columbia University, New York, NY, ⁵Department of Pediatrics, Columbia University, New York, NY
*Purpose: Alloantibodies produced by recipient B cells are associated with rejection after intestinal transplantation (ITx) and negatively impact patient and graft survival. However, the dynamics…
2019 American Transplant Congress
CyTOF and RNAseq Analyses Unravel Early Phenotypic and Transcriptional Changes in Antibody-Secreting and Memory B Cells >1 Year Prior to Antibody-Mediated Rejection
C. Fischman¹, M. Fribourg¹, J. Ansari², L. Gallon², F. Ginevri³, P. Comoli⁴, M. Cioni³, A. Nocera³, M. Bruschi³, C. Cantarelli¹, L. Perin⁵, P. Cravedi¹
¹Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, ²Department of Medicine, Northwestern University, Chicago, IL, ³Department of Medicine, IRCCS Istituto G. Gaslini, Genoa, Italy, ⁴Department of Medicine, IRCCS San Matteo Hospital, Pavia, Italy, ⁵Department of Urology, Children's Hospital Los Angeles, Los Angeles, CA
*Purpose: While the negative impact of anti-HLA donor specific antibodies (DSA) on graft outcomes has been extensively investigated, little is known about early changes in…
2019 American Transplant Congress
ICOS⁺PD-1⁺ Circulating T Follicular Helper Cells Are a Biomarker of Humoral Alloreactivity and Predict Donor-Specific Antibody Generation Following Transplantation
G. M. La Muraglia II, M. E. Wagener, M. L. Ford, I. R. Badell
Emory Transplant Center, Atlanta, GA
*Purpose: Mounting evidence in kidney transplantation suggests that donor-specific antibodies (DSA) significantly contribute to renal allograft loss. However, biomarkers to guide clinical management of DSA…
2019 American Transplant Congress
Clazakizumab (CLZ, Anti-IL-6 Antibody) and Tocilizumab (TCZ, Anti-IL-6 Receptor [r] Antibody) Treatments Differentially Affect IL-6/IL-6R Signaling by Modulating Soluble IL-6R (sIL-6R) and gp130 (sgp130) in Kidney Transplant Patients (KTx Pts) Treated for Chronic Antibody-Mediated Rejection (cABMR)
B. Shin, S. Ge, A. Petrosyan, N. F. Ammerman, A. A. Vo, S. C. Jordan, M. Toyoda
Cedars-Sinai Medical Center, Los Angeles, CA
*Purpose: We have reported successful use of TCZ for cABMR treatment in HLA-sensitized KTx Pts. CLZ is 3-120 times more potent than TCZ in IL-6/IL-6R…
2019 American Transplant Congress
Sequential Dosing of Rituximab and IVIg for Desensitization of Highly-HLA Sensitized (HS) Patients Awaiting HLAi Kidney Transplantation
A. A. Vo, E. Huang, S. Williamsons, C. Myers, A. Peng, R. Najjar, S. Sethi, K. Lim, N. Ammerman, S. C. Jordan
Kidney Transplant, Cedars Sinai Medical Ctr, Los Angeles, CA
*Purpose: Recent observations suggest that the use of IVIg + rituximab has a critical role in modifying allo-reactive B-cells prior to HLAi kidney transplantation, thus…
2019 American Transplant Congress
Resolution of Hyperglycemia Following Islet Allograft Transplantation in Non-Immunosuppressed Mice
J. Ge¹, K. Lee¹, C. Yang¹, R. White², I. Rosales², P. Russell¹, C. Rickert¹, J. Markmann¹, J. Madsen¹, R. Colvin², A. Alessandrini¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Pathology, Massachusetts General Hospital, Boston, MA
*Purpose: Kidney allografts are naturally tolerogenic in mice. Certain mouse strain combinations develop tolerance of kidney allografts across full MHC incompatibilities without immunosuppression (e.g. DBA/2…