Articles tagged "Apoptosis"

2020 American Transplant Congress
Protective Effect of Fucoidan Against Tacrolimus-Induced Nephrotoxicity in LLC-PK1 Cells
H. J. Jang
Surgery, University of Ulsan, Gangneung Asan Hospital, Gangneung, Korea, Republic of
*Purpose: Tacrolimus (FK506) is an immunosuppressant agent that is frequently used to prevent rejection of solid organs upon transplant. However, nephrotoxicity due to apoptosis and…
2020 American Transplant Congress
Immunomodulation with FasL Presenting Biomaterials Achieves Long-Term Survival of Allogeneic Islets in the Absence of Chronic Immunosuppression in a Non-Human Primate Model
J. Lei¹, H. Deng¹, T. Chen¹, K. Lee¹, C. Peters¹, Z. Yang¹, K. Deng¹, G. Rickert¹, I. Rosales¹, N. Serifis¹, M. Coronel², M. Hunckler², E. Yolcu³, H. Shirwan³, A. García², J. Markmann¹
¹Surgery, Mass General, Boston, MA, ²School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, ³School of Mechanical Engineering, University of Louisville School of Medicine, Louisville, KY
*Purpose: The full potential of islet transplantation will only be realized by the development of tolerogenic strategies that obviate the need for maintenance immunosuppressants. We…
2020 American Transplant Congress
Caspase-1 Knockout Mice Have Increased Survival after Cold Ischemia in a Murine Kidney Transplant Model
S. Jain¹, R. Plenter², T. Nydam², A. Jani¹
¹Renal, University of Colorado, Aurora, CO, ²Transplant Microsurgery Program, University of Colorado, Aurora, CO
*Purpose: Prolonged cold ischemia (CI) is a known risk factor for acute kidney injury (AKI) after kidney transplantation. However, the mechanism by which CI leads…
2020 American Transplant Congress
Endothelial Cells Internalize Apoptotic Exosome-Like Vesicles Through Phosphatidylserine-Dependent Macropinocytosis
F. Migneault, M. Dieudé, A. Brodeur, N. Thibodeau, D. Beillevaire, M. Hébert
CRCHUM, Montreal, QC, Canada
*Purpose: Ischemia-reperfusion and rejection favor endothelial apoptosis, which in turn contributes to maladaptive vascular repair leading to transplant vasculopathy. Apoptotic endothelial cells (EC) release exosome-like…
2020 American Transplant Congress
3D Bioprinted Intrahepatic Cholangiocarcinoma Model and 7-Day Perfusion with Gemcitabine and Cisplatin: Modeling the Tumor Microenvironment and Response to Chemotherapy
A. M. Chen¹, W. Zhang¹, J. Sterner², L. Kennedy³, A. Isidan¹, E. Gramelspacher¹, H. L. Francis³, G. Alpini³, L. J. Smith², P. Li¹, B. Ekser¹
¹Division of Transplant Surgery - Indiana University, Indianapolis, IN, ²3D Bioprinting Core and Department of Radiology and Imaging Sciences - Indiana University, Indianapolis, IN, ³Division of Gastroenterology, Richard L. Roudebush VA Medical Center and Indiana University, Indianapolis, IN
*Purpose: Over 8,000 patients are newly diagnosed with cholangiocarcinoma (CCA) in the United States each year.Understanding the CCA microenvironment will facilitate the development of novel…
2020 American Transplant Congress
Apoptotic Exosome-Like Vesicles are Associated with Alloimmune Vascular Injury in Kidney Transplant Recipients
M. Dieude¹, E. Boilard², I. Allaeys², A. Deneault-Marchand¹, J. Turgeon¹, E. Dore², S. Lariviere-Beaudoin¹, A. Karakeussian-Rimbaud¹, S. Collette³, L. Senecal³, M. Hebert¹, H. Cardinal¹
¹Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, QC, Canada, ²Centre de Recherche du Centre Hospitalier de l'Université de Québec, Quebec, QC, Canada, ³Hopital Maisonneuve-Rosemont, Montreal, QC, Canada
*Purpose: There is no non-invasive biomarker of alloimmune vascular injury. We have previously characterized exosome-like vesicles enriched in active proteasome and LG3 (ApoExo) that are…
2020 American Transplant Congress
Activation of Tacrolimus Induced Cytotoxicity Through Apoptotic Pathways
S. Chung, S. Choi
Department of Surgery, Chonnam National University, Gwangju, Korea, Republic of
*Purpose: To elucidate the mechanism of cytotoxicity in FK506-treated Jurkat T cells, signal transduction pathway of TNF-related events was studied.*Methods: Viability of Jurkat T cells…
2020 American Transplant Congress
The Inflammatory Response in the Context of Tissue Injury: Roles of Tyro3, Axl, and Mer (TAM) Receptors in Liver Ischemia/Reperfusion Injury
J. Zhang, M. Ni, D. Jin, H. Zhang, R. Busuttil, J. Kupiec-Weglinski, Y. Zhai
Dumont-UCLA Transplant Center, Los Angeles, CA
*Purpose: Although the inflammatory response in liver ischemia reperfusion injury has been studied extensively, how the context of tissue injury (presence of necrotic/apoptotic cells) regulates…
2020 American Transplant Congress
Lack of Dj-1 Amplifies Sepsis-Associated Acute Kidney Injury and Increases Parenchymal Apoptosis
J. Leeds¹, Y. Scindia², E. Ghias¹, S. Cechova¹, V. Loi¹, E. Mandziak¹, S. Swaminathan¹
¹University of Virginia, Charlottesville, VA, ²University of Florida, Gainesville, FL
*Purpose: Sepsis following kidney transplantation is a frequent complication due to mandatory immunosuppression and increased infection risk. Kidney transplant recipients who develop sepsis show inferior…
2020 American Transplant Congress
Torc1 Inhibition Protects Activated Human Regulatory T Cells from Self-Inflicted Damage via Granzyme B
J. B. Alhaddad, H. Allos, B. S. Al Dulaijan,, S. K. Eskandari, I. Sulkaj, G. Melhem, J. Y. Choi, J. R. Azzi
Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School, Boston, MA
*Purpose: The use of Regulatory T cells (Tregs) in adoptive cell therapy has been hindered by their short survival following their adoptive transfer. We have…