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What Threshold MFI is the Luminex 1:8 Dilution the Poor Man's C1q for Patients Awaiting Heart Transplant?

M. Kittleson, J. Patel, E. Kransdorf, R. Levine, S. Dimbil, D. Geft, D. Chang, L. Czer, J. Kobashigawa.

Cedars Sinai Medical Center, Los Angeles.

Meeting: 2018 American Transplant Congress

Abstract number: B43

Keywords: HLA antibodies, Outcome, Sensitization

Session Information

Session Name: Poster Session B: Heart and VADs: All Topics

Session Type: Poster Session

Date: Sunday, June 3, 2018

Session Time: 6:00pm-7:00pm

 Presentation Time: 6:00pm-7:00pm

Location: Hall 4EF

Purpose: Heart transplant candidates are evaluated for anti-HLA antibodies by Luminex single-antigen bead testing to avoid donors that may harbor antigens that these antibodies are against. To identify strongly-binding antibodies, the Luminex assay can be performed via a 1:8 dilution of the patient's serum. In addition, a C1q assay can be used to measure the ability of an antibody to bind complement and therefore have the potential to cause injury to a potential donor heart. It has been reported that the antibody binding strength described as mean fluorescent intensity (MFI) does not correlate to complement binding. However, 1:8 dilution may correlate to complement binding. We sought to assess whether there is a correlation between the Luminex 1:8 dilution assay and a positive C1q assay in patients awaiting heart transplant. This is important as many programs do not have the C1q assay available.

Methods: Between 2010 and 2016, we identified 42 patients with anti-HLA antibody on the Luminex 1:8 assay and divided them into groups of MFI > 2500, 5000 and 10000). We then evaluated these samples for complement fixation (C1q assay) on the same date of serum analysis.

Results: 32/51 patients (62.7%) with MFI >2500, 36/42 (85.7%) patients with MFI>5000, and 10/11 (90.9%) of patients with MFI>10000 on 1:8 dilution had positive C1q fixation. There was a significant difference between the three groups with respect to complement fixation (p=0.017).

Conclusion: The proportion of patients with positive complement fixation is significant for MFI >2500 which is the lowest MFI detected on our Luminex 1:8 dilution. Thus, it appears that detection of antibody with the Luminex 1:8 dilution is the poor man's C1q assay. The corresponding antigens for these anti-HLA antibodies should be avoided in a virtual crossmatch for these patients awaiting heart transplantation.

Thresholds on Luminex 1:8 Assay
MFI > 2500 (n=51) MFI >5000 (n=42) MFI > 10000 (n=11) P-Value
% Complement Fixation 62.7% (32/51) 85.7% (36/42) 90.9% (10/11) 0.017

CITATION INFORMATION: Kittleson M., Patel J., Kransdorf E., Levine R., Dimbil S., Geft D., Chang D., Czer L., Kobashigawa J. What Threshold MFI is the Luminex 1:8 Dilution the Poor Man's C1q for Patients Awaiting Heart Transplant? Am J Transplant. 2017;17 (suppl 3).

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To cite this abstract in AMA style:

Kittleson M, Patel J, Kransdorf E, Levine R, Dimbil S, Geft D, Chang D, Czer L, Kobashigawa J. What Threshold MFI is the Luminex 1:8 Dilution the Poor Man's C1q for Patients Awaiting Heart Transplant? [abstract]. https://atcmeetingabstracts.com/abstract/what-threshold-mfi-is-the-luminex-18-dilution-the-poor-mans-c1q-for-patients-awaiting-heart-transplant/. Accessed June 4, 2025.

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