ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

The Property of Modulatory T Cells Generated by Mixed Lymphocyte Culture with CD154 Blockade.

K. Hotta, T. Oura, B. Cosimi, T. Kawai.

Center for Transplantation Science, Massachusetts General Hospital, Boston, MA.

Meeting: 2016 American Transplant Congress

Abstract number: A27

Keywords: Alloantigens, CD4, Co-stimulation, T helper cells

Session Information

Session Name: Poster Session A: B cells & AMR, Alloreactivity, Immune Regulation & Regulatory T Cells, T Cell Biology and Alloreactivity, Immunesuppression

Session Type: Poster Session

Date: Saturday, June 11, 2016

Session Time: 5:30pm-7:30pm

 Presentation Time: 5:30pm-7:30pm

Location: Halls C&D

Background:Regulatory T cell (Treg) immunotherapy has been applied to minimize the immunosuppressive drugs in clinical transplantation. To avoid contamination of CD4+CD25- T cells, only highly purified Tregs have been infused to the patients. In this study, we investigated immunological properties of CD4+CD25- T cells after mixed lymphocyte culture (MLC) with a costimulatory blockade.

Method: Isolated CD4+ T cells (Responder A) from human blood buffy coat were cultured with irradiated allogeneic PBMCs (Stimulator B) for 14 days in the presence or absence of anti-human CD154 mAb. These primed CD4+ T cells were then added as a modulator (Modulator A) to a newly prepared (second) MLC with Responder A and irradiated original stimulator (Simulator B) or third party stimulator (Stimulator C) for 5 days.

Results: There was no significant difference in the percentage of Tregs induced after MLC with or without aCD154mAb. However, Modulator A generated after MLC with aCD154mAb significantly more suppressed the second MLC than Modulator A generated without aCD154mAb. These modulators generated with aCD154mAb also displayed antigen-specific suppression as Modulator A generated by Stimulator B with anti-CD154 mAb in the first MLC significantly more suppressed the second MLC with Stimulator B than with Stimulator C (Fig. 1). To identify which T cell subset possesses suppressive function, CD4+CD25highFoxP3+ cells (Treg) and CD4+CD25-FoxP3- cells (non-Treg) were sorted after the first MLC. Treg isolated after the first MLC displayed significant suppressive function of the second MLC. Interestingly, although suppressive function was not prominent as Treg, non-Treg isolated after the first MLC also displayed suppressive function (Fig. 2).

Conclusion: These data indicate that suppressive function can be expected even with antigen-primed non-Treg. In vivo studies to evaluate a suppressive function of these non-Tregs are currently underway.

CITATION INFORMATION: Hotta K, Oura T, Cosimi B, Kawai T. The Property of Modulatory T Cells Generated by Mixed Lymphocyte Culture with CD154 Blockade. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

To cite this abstract in AMA style:

Hotta K, Oura T, Cosimi B, Kawai T. The Property of Modulatory T Cells Generated by Mixed Lymphocyte Culture with CD154 Blockade. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/the-property-of-modulatory-t-cells-generated-by-mixed-lymphocyte-culture-with-cd154-blockade/. Accessed June 1, 2025.

« Back to 2016 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences