Objective Research of semimature dendritic cell(smDCs) inducing transplantation toleranceof hepatocytes differentiated from mouse embryonic stem cells (ESCs) and its mechanismtions.

Methods Inducing 129 mouse ESCs labelled green fluorescent protein(GFP) into hepatocytes as early methods. Meanwhile, inducing bone marrow mononuclear cells of 129 mouse into smDCs and regulatory dendritic cells(regDCs). Moreover, the hepatocytes differentiated from 129 mouse ESCs were transplanted into liver of BALB/c mouse 3 days after infusing smDCs and regDCs suspension of 129 mouse into BALB/c mouse by tail vein respectively. After that, the growth status and survival time of transplanted cells in the recipient and infiltration of lymphocytes in transplant parts were observed. Furthermore, Foxp3 expression of peripheral blood CD4+T cells were also tested.

Results In the control group, the transplanted cells in liver of BALB/c mouse could survive about 1 week only. In contrast, the transplanted cells of smDC groups and regDCs groups could survive about 4 weeks and the transplant parts of smDC groups also had less CD3+ T cells. The morphology of smDCs were similar with regDCs. The expression of MHC-II,CD40,CD80 and CD86 on smDCs and regDCs were moderate.Moreover, the Foxp3 expression of peripheral blood CD4+T cells in smDC groups were higher than that in the control groups, from 1.11% up to 5.38%. The Foxp3 expression in regDC groups rose to 3.87%.

Conclusion The smDCs could induce transplantation tolerance of hepatocytes differentiated from 129 mouse ESCs in the recipient.The mechanism was associated with high level of Foxp3+Tregs, which could be increased by means of smDCs appropriate expression of MHC-II,CD40,CD80 and CD86 . The smDCs and regDCs were the same type of tolerance dendritic cells.

CITATION INFORMATION: Hu A, Zhang C, Liao W, Zhu X, He X. The Effect of Semimature Dendritic Cell on Transplantation Tolerance of Hepatocytes Differentiated from Mouse Embryonic Stem Cells. Am J Transplant. 2017;17 (suppl 3).

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