Objective The ischemia/reperfusion (I/R) injury plays an important role in delayed graft function (DGF) after kidney transplantation, however, the mechanism is not fully understood. Therefore, identifying new regulatory genes in the I/R injury is helpful for the prevention and treatment of DGF.

Methods C57BL/6 mice undergone renal I/R injury operation were used in the study, and sham operated mice were employed as a negative control. The renal function was reflected by the serum creatinine levels. The profile of differential expression genes was screened using a microarray. The expression of Tax1bp3 in the kidney was detected using immunochemistry staining and western blotting. The interaction between Tax1bp3 and LZAP was detected using immunoprecipitation.

Results The renal I/R injury operation induced a significant increase of serum creatinine compared with the sham operated mice. From the data of microarray, we identified that the expression of Tax1bp3 was increased to 2.3 fold of the control mice. Consistently, the protein levels were upregulated in the I/R injured kidney, which indicated that Tax1bp3 might play a regulatory effect in I/R injury. By using immunoprecipitation assays, we observed that there is an interaction between Tax1bp3 and LZAP. The latter is a known factor that could positively active apoptosis. These results indicated that the increased expression of Tax1bp3 in I/R injured kidney might modulate apoptosis through an LZAP-dependent manner.

Conclusion The expression of Tax1bp3 was upregulated in I/R injured kidney, which may be a potential therapeutic target for the I/R-induced DGF.

CITATION INFORMATION: Qiu W., Lin J., Tian Y. Tax1bp3 is Upregulated in the Ischemia/Reperfusion Injury of Kidney Am J Transplant. 2017;17 (suppl 3).

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