ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Simultaneously Detection of ddcfDNA in Plasma and Urine Assist to Determine the Type of Rejection in Renal Transplant Patients

D. Cheng1, F. Liu2, H. Liu2, T. Jiang2, Y. Zhou3, L. Shu2, X. Li1, K. Xie1, X. Ni1, J. Chen1, J. Ge2, Z. Liu1, H. Shi3

1Jinling Hospital Nanjing University, Nanjing, China, 2AlloDx, Shanghai
Suzhou, China, 3Jiangsu University, Zhenjiang, China

Meeting: 2020 American Transplant Congress

Abstract number: B-322

Keywords: Antibodies, Kidney, Rejection, T cells

Session Information

Date: Saturday, May 30, 2020

Session Name: Poster Session B: Biomarkers, Immune Assessment and Clinical Outcomes

Session Time: 3:15pm-4:00pm

 Presentation Time: 3:30pm-4:00pm

Location: Virtual

Related Abstracts
  • The Plasma DdcfDNA Level Associates with the Pathological Characteristics in Renal Allograft
  • Periphery Blood ddcfDNA Can Significantly Differentiate Acute Humoral Rejection from Banff’s Type I Acute Rejection in Kidney Transplantation

*Purpose: The correct diagnosis and treatment of acute rejection (ABMR and TCMR) are the key to the long-term survival after renal transplantation. Renal biopsy is a gold standard for diagnosis of ABMR and TCMR. Donor-derived cell-free DNA (ddcfDNA) detected in transplant recipients has been reported as a noninvasive marker to diagnose allograft injury. Pathological analysis show that ABMR and TCMR have different injury sites for the graft, the ABMR mainly infects vascular endothelial cells and apoptotic endothelial cells release cfDNA into blood stream directly. Meanwhile, TCMR-associated injury that principally occurs in the tubulointerstitial compartment and apoptotic epithelial cells release cfDNA into urine directly. Herein, the purpose of this study was to perform ddcfDNA analysis of blood and urine to determine the type of rejection in renal transplant patients.

*Methods: DdcfDNA was assayed in plasma and corresponding urine samples from 56 recipients whose renal biopsy proved acute rejection. We measured the fraction of ddcfDNA in plasma and the absolute amount of ddcfDNA in urine (in-house data show absolute quantity of ddcfDNA have more significant in urine). ddcfDNA(%) quantification through target region capture sequencing and calculated by maximum likehood estimation (MLE).

*Results: Allograft pathology was classified according to the Banff 2013, including 25 patients with biopsy-proven ABMR, 21 patients with TCMR IA/IB and 10 patients with TCMR IIA/IIB. All samples have no BKV detected in urine or blood. Analysis ddcfDNA in plasma , the median ddcfDNA level of ABMR group was 1.43% (IQR 1.18%-2.54%), TCMR IA/IB group was 0.93%(IQR 0.74%-1.74%), TCMR IIA/IIB group was 1.81% (IQR 1.39%-4.33%). Significant differences between IA/IB group and ABMR group (p=0.013)/IIA/IIB group(p=0.01) was found, but no significant difference between ABMR group and IIA/IIB group (p>0.05). Analysis ddcfDNA in urine, the median ddcfDNA(ng/mL) of ABMR, TCMR IA/IB and TCMR IIA/IIB group was 2.6ng/mL (IQR 2.02ng/mL-3.82ng/mL), 4.98ng/mL (IQR 3.82ng/mL-7.09ng/mL), 7.01ng/mL (IQR 5.22ng/mL-9.39ng/mL), respectively. There were significant differences among the three groups (p<0.001; p<0.001; p=0.047). (Figure 1).(ABMR, TCMR I/II).

 border=

*Conclusions: Our results indicated that simultaneous detection ddcfDNA in blood and urine can distinguish the type of rejection of renal transplantation.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Cheng D, Liu F, Liu H, Jiang T, Zhou Y, Shu L, Li X, Xie K, Ni X, Chen J, Ge J, Liu Z, Shi H. Simultaneously Detection of ddcfDNA in Plasma and Urine Assist to Determine the Type of Rejection in Renal Transplant Patients [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/simultaneously-detection-of-ddcfdna-in-plasma-and-urine-assist-to-determine-the-type-of-rejection-in-renal-transplant-patients/. Accessed February 27, 2021.

« Back to 2020 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.