ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Rhesus-Primatized Mouse Model Generated Using Transgenic Immunodeficient Mice

C. Little1, W. J. Haynes1, L. Huang1, C. Daffada1, K. B. Wolfe2, E. Perrin2, J. A. Simpson1, J. A. Kropp Schmidt2, H. M. Hinkle2, L. T. Keding2, R. T. Behrens2, D. T. Evans2, D. Kaufman1, J. A. Thomson2, T. G. Golos2, M. Brown1

1Surgery, University of Wisconsin, Madison, WI, 2University of Wisconsin, Madison, WI

Meeting: 2022 American Transplant Congress

Abstract number: 178

Keywords: Engraftment, Mice, Primates, Stem cells

Topic: Basic Science » Basic Science » 05 - Translational Cellular Therapies: Islet and Stem Cell Transplantation

Session Information

Session Name: Cellular/Islet Therapies and Tissue Engineering

Session Type: Rapid Fire Oral Abstract

Date: Sunday, June 5, 2022

Session Time: 5:30pm-7:00pm

 Presentation Time: 5:50pm-6:00pm

Location: Hynes Room 310

*Purpose: To develop a translational primatized mouse model, akin to humanized mice, that allows for high-throughput in vivo experimentation to augment non-human primate pre-clinical studies.

*Methods: Bead-separated CD34+ rhesus macaque cells (1×105-1×106) were intravenously transplanted into strains of immunodeficient mice crossbred with human cytokine transgenes. Autologous thymic fragments were surgically placed within the mouse kidney capsule to allow for de novo T cell development. Rhesus cross-reactive anti-CD2 was administered for passenger leukocyte depletion. Flow cytometric analyses were used for chimerism testing and immunoprofiling. Mixed lymphocyte reactions and SIVmac239 infection assays were employed for in vitro allogenic and infectious functional analysis, respectively.

*Results: Fetal liver-derived hematopoietic stem and progenitor cells (HSPCs) were found to have higher relative CD34hi fractions (containing progenitors with the greatest engraftment potential) compared to adult mobilized blood and bone marrow. Co-transplantation of fetal liver-derived CD34-enriched HSPCs and autologous thymic fragments into immunodeficient mice harboring transgenes for human (rhesus cross-reactive) GM-CSF and IL-3 yielded robust, multilineage lymphohematopoietic engraftment (Figure 1A). The resulting emergent immune system recapitulated that of the HSPC source, with similar relative frequencies of leukocyte subsets within the thymic, secondary lymphoid, and peripheral compartments. Importantly, despite exhibiting a predominantly naive phenotype across all immune cell types, in vitro functional assays demonstrated robust cellular activation in response to non-specific and allogenic stimuli (Figure 1B). Further, primatized mouse splenocytes supported in vitro SIV infection, which yielded a robust viral replication curve.

*Conclusions: We have developed a durable primatized mouse model, characterized by robust multilineage engraftment yielding a functional macaque immune system that is well-poised for developmental and immunological studies.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Little C, Haynes WJ, Huang L, Daffada C, Wolfe KB, Perrin E, Simpson JA, Schmidt JAKropp, Hinkle HM, Keding LT, Behrens RT, Evans DT, Kaufman D, Thomson JA, Golos TG, Brown M. Rhesus-Primatized Mouse Model Generated Using Transgenic Immunodeficient Mice [abstract]. Am J Transplant. 2022; 22 (suppl 3). https://atcmeetingabstracts.com/abstract/rhesus-primatized-mouse-model-generated-using-transgenic-immunodeficient-mice/. Accessed May 9, 2025.

« Back to 2022 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences