ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Reactivation of Murine Polyomavirus Replication by Renal Ischemia-Reperfusion Injury and Immunosuppression

G. Huang1, Y. Huang1, X. Chen1, J. Qiu1, J. Li1, G. Li2, C. Wang1, L. Chen1

1The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China, 2Guangdong Key Laboratory of Laboratory Animal, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China

Meeting: 2020 American Transplant Congress

Abstract number: A-222

Keywords: Immunosuppression, Mice, Polyma virus, Renal ischemia

Session Information

Session Name: Poster Session A: Kidney: Polyoma

Session Type: Poster Session

Date: Saturday, May 30, 2020

Session Time: 3:15pm-4:00pm

 Presentation Time: 3:30pm-4:00pm

Location: Virtual

*Purpose: Polyomavirus (PyV) nephropathy is an important cause of graft failure after renal transplantation. Its pathogenesis is associated with immunosuppression (IM) and renal ischemia-reperfusion injury (IRI). We sought to test this notion experimentally.

*Methods: Mouse PyV (MPyV) LID-1 strain was propagated in NIH 3T3 cells and 105 genomic equivalents were used to infect male mice by the intraperitoneal route one day after birth resulting in primary infection. 9 weeks later, they were divided into three groups. In the renal IRI group, the left renal pedicle was clamped for 30 minutes and then released. In the IM group, the mice were treated with Tacrolimus (2.5 mg/kg) and Mycophenolate mofetil (50 mg/kg) by the intragastric route daily, and those in the control group did not receive any treatment. Viral load kinetics was studied by qPCR in groups of 4 mice, sacrificed 3, 7, 10, 14 and 21 after corresponding treatment. High-throughput RNA-seq and pathological HE staining were performed on the renal tissue.

*Results: MPyV IgG in serum were positive and DNA load in plasma was less than 102 copies/ul before experiment in all 9 week old mice. Viral load in the left kidney was significantly 10 fold higher than that in the right kidney on the 3rd day after IRI (P=0.0013), then MPyV DNA load in both side of the kidney decreased with no remarkable difference at other time points (all P>0.05). Viral load was similar between the left and right kidney (P>0.05) on the 3rd day after immunosuppression. It was also similar to the right kidney (P>0.05) but lower than the left kidney in IRI group, and did not decrease with time. The gene ontology functional enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that there were significant differences in viral carcinogenesis, NF-kB signaling pathway and complement and coagulation cascades between the left and right kidneys on the 3rd day.

*Conclusions: MPyV, which is latent after primary infection can be reactivated and maintained a high level in kidney through renal IRI and immunosuppression.

 border=

 border=

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

To cite this abstract in AMA style:

Huang G, Huang Y, Chen X, Qiu J, Li J, Li G, Wang C, Chen L. Reactivation of Murine Polyomavirus Replication by Renal Ischemia-Reperfusion Injury and Immunosuppression [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/reactivation-of-murine-polyomavirus-replication-by-renal-ischemia-reperfusion-injury-and-immunosuppression/. Accessed June 1, 2025.

« Back to 2020 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences