Date: Saturday, June 11, 2016
Session Name: Poster Session A: Kidney: Acute Cellular Rejection
Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Halls C&D
Plasma cells (PC) are usually defined functionally as antibody secreting cells (ASC). Depending upon their life span, they could either be short lived or long lived. Long lived PC (LLPC), immediately after differentiation, migrate to survival niches within the bone marrow or spleen and are responsible for long term production of antibody. We intend to correlate the concentration of peripheral LLPC with that of acute rejection in patients with Kidney transplantation.
Peripheral venous blood was collected from patients before transplant (Pre- Tx) and after transplant (Post-Tx) at post-1 month, 3 month, 6 month and 12 months and at the time of rejection. Peripheral plasma cells were calculated by flowcytometry using MAb against CD20, CD38 and CD138. CD20-CD38+CD138+ long lived plasma cells were calculated as a % fraction of total lymphocytes.
This study includes 78 first kidney transplant recipients who were divided into two groups: Rejection group (R, n=38) and Non Rejection group (NR, n=40). The mean onset of rejection was 28.6 days (Range 3- 182 days). Therefore the LLPC level during rejection was compared with post- 1 month peripheral level of LLPC of patients under group NR. Age and sex matched healthy donors prior to nephrectomy were recruited as control (n= 40). P value <0.05 was taken as significant.
Patients with end stage renal disease didn't have significant difference in the peripheral LLPC level as compared to control (P=0.7711). Post- 1 month of transplant we could not demonstrate significant alteration in the LLPC level in patients with stable graft function (2.713 ± 0.1790 vs. baseline 2.545 ± 0.1370, P=0.2690). However, during rejection we were able to demonstrate significant decrease in PC level from the peripheral circulation (0.7447 ± 0.09584 vs. baseline 2.363 ± 0.1529, P<0.0001).
Decrease in the long lived plasma cells from peripheral circulation is associated with acute rejection. Therefore, study of LLPC could be incorporated when prediction model is to be designed for acute rejection in Kidney transplantation.
CITATION INFORMATION: Minz R, Dhital R, Minz M, Nada R, Sharma A, Singh S, Kenwar D. Post-Transplant Reduction of Long Lived Plasma Cells from Peripheral Circulation Is Associated with Acute Rejection in Kidney Transplant Recipients. Am J Transplant. 2016;16 (suppl 3).
To cite this abstract in AMA style:Minz R, Dhital R, Minz M, Nada R, Sharma A, Singh S, Kenwar D. Post-Transplant Reduction of Long Lived Plasma Cells from Peripheral Circulation Is Associated with Acute Rejection in Kidney Transplant Recipients. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/post-transplant-reduction-of-long-lived-plasma-cells-from-peripheral-circulation-is-associated-with-acute-rejection-in-kidney-transplant-recipients/. Accessed January 28, 2021.
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