ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Novel Flow Cytometry Method for Detecting Complement-Fixing Blood Group IgG Antibody in ABO-Incompatible Renal Transplantation

T. Ishizuka,1 M. Yasuo,1 Y. Ishida,1 H. Miura,1 K. Iwadoh,2 T. Murakami,2 K. Kitajima,2 I. Nakajima,2 S. Fuchinoue.2

1Transplant Immunology, Tokyo Women's Medical University, Shinjuku ward, Tokyo, Japan
2Surgery III, Tokyo Women's Medical University, Shinjuku ward, Tokyo, Japan.

Meeting: 2015 American Transplant Congress

Abstract number: A133

Keywords: Antibodies, FACS analysis, Kidney transplantation

Session Information

Session Name: Poster Session A: Kidney Antibody Mediated Rejection

Session Type: Poster Session

Date: Saturday, May 2, 2015

Session Time: 5:30pm-7:30pm

 Presentation Time: 5:30pm-7:30pm

Location: Exhibit Hall E

【Background】We experienced a hyperacute rejection (HAR) in ABO-incompatible renal transplantation (ABO-I RTx) on POD 0. We hypothesized that complement-fixing IgG anti-A/B blood group antibody (anti-A/B Ab) played a critical role in developing antibody mediated acute rejection (AMAR) in ABO-I RTx and developed a novel flow cytometry method for complement C1q (FCM-C1q) to detect it.

【Method】We selected a few ABO-I RTx recipients who developed severe HAR or severe AMAR. We then tested their stored pre/post-transplant serum for C1q level on anti-A/B Ab by FCM-C1q.

【Results】A 60-year old female underwent ABO-I RTx. No immunological risk was detected by conventional tests before surgery with anti-A Ab titer ×32 on POD 0. She, however, developed so severe a HAR during the operation that there was no other way than to perform graftectomy. Her FCM-C1q level on –2POD was as high as 68.1%. A 58-year old male underwent ABO-I RTx. All pre-transplant immunological tests were negative with anti-A Ab titer ×32 on POD 0. Then s-Cr began to rise on POD 7 developing severe AMAR and we were forced to conduct graftectomy on POD 10. His FCM-C1q level on –8POD was 75.2%. Although it had fallen down to 0.616% on POD 4 probably due to preoperative plasma exchanges, it gradually turned to rise after POD 7. A 39-year old male underwent ABO-I RTx. He developed severe humoral rejection (HR) on POD 48 and lost graft on POD 51.His FCM-C1q levels stayed < 1% until POD25. It rose to 4.47% and a biopsy on POD 28 showed borderline HR for the first time. It went up to > 10% after POD35 and moderate HR with intravascular coagulation was found in a biopsy on POD 48. He lost graft on POD51 with FCM-C1q level 40.5% on POD 50.

【Conclusion】Our case study with FCM-C1q suggested a possibility that presence of complement-fixing anti-A/B Ab was more essential in developing AMAR and HAR in ABO-I RTx than a whole level of anti-A/B Ab titer.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Ishizuka T, Yasuo M, Ishida Y, Miura H, Iwadoh K, Murakami T, Kitajima K, Nakajima I, Fuchinoue S. Novel Flow Cytometry Method for Detecting Complement-Fixing Blood Group IgG Antibody in ABO-Incompatible Renal Transplantation [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/novel-flow-cytometry-method-for-detecting-complement-fixing-blood-group-igg-antibody-in-abo-incompatible-renal-transplantation/. Accessed May 8, 2025.

« Back to 2015 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences