ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Monocytic TNF-α Response to EBV Peptides as a Mean to Identify Over-Immunosuppressed Kidney Transplant Recipients.

P. Vallin,1 O. Désy,1 S. Béland,1 E. Wagner,2 S. De Serres.1

1Renal Division, Quebec University Health Center, Laval University, Quebec, QC, Canada
2Immunology and Histocompatibility Laboratory, Quebec University Health Center, Laval University, Quebec, QC, Canada.

Meeting: 2016 American Transplant Congress

Abstract number: A275

Keywords: Epstein-Barr virus (EBV), Immunosuppression, Tumor necrosis factor (TNF)

Session Information

Date: Saturday, June 11, 2016

Session Name: Poster Session A: Poster Session III: Kidney Complications-Other

Session Time: 5:30pm-7:30pm

 Presentation Time: 5:30pm-7:30pm

Location: Halls C&D

Related Abstracts
  • Inverse Monocytic Subset Profile in Blood and Tissue During Heart Transplant Rejection
  • Increased Precentage of Circulating CD16+ Monocytes Before Transplantation Are Associated With the Occurence of Acute Rejection

Background. The reduction in acute rejection rates seen with the current triple immunosuppressive maintenance regimen has come at the expense of an increased risk of serious infections and cancer. A clinical tool to quantify the level of immunosuppression would be key to prevent these undesirable events.

Methods. This is an interim report of an ongoing prospective, observational longitudinal single center study of 50 de novo kidney recipients in which PBMC were collected at 12 timepoints over 3 yrs post transplant (600 samples). The immunosuppression regimen consisted of basiliximab followed by prednisone, tacrolimus and mycophenolate. Patients were classified as over-immunosuppressed (OIS: opportunistic infection, BK viremia or nephropathy, CMV viremia, ≥3 recurrent bacterial infection) or controls. We examined IFNγ response in CD4+, CD8+ T cells and NK cells, as well as TNFα response in monocyte subsets by intra/extracellular flow cytometry. Early in the discovery phase, results in the first 7 patients indicated that TNFα response to EBV peptides in CD14+CD16+ monocytes was lower in OIS patients. This was further evaluated in 21 patients using the first 2 years samples (0, 1, 3, 6, 9, 12, 16, 20 and 24mo). The analysis was conducted using mixed models, ROC curve, sensitivity/specificity and PPV/NPV.

Results. We studied 8 OIS patients and 13 controls. Overall, the mean(±std dev) TNFα-positive CD14+CD16+ monocytes was 58±24 vs. 79±17 percent in OIS and controls patients respectively (p=0.004), with more stable values in controls (within-patient mean std dev=10 vs. 19). A ROC curve built across all timepoints resulted in a threshold of 69 percent of TNFα-positive monocytes (AUC=0.74, p<0.001). A classifier rule based on ≥2 consecutive values below this threshold provided a sensitivity and specificity of 88% (7/8) and 77% (10/13) respectively, with positive and negative predictive values of 70% and 91% respectively. Analysis of effector and naive T cell IFNγ responses showed no correlation with the OIS phenotype.

Conclusion. This longitudinal analysis of the cellular immune profile suggest that stimulation of PBMC with EBV peptides allows to identify OIS patients, through the measurement of TNFα response in CD14+CD16+ monocytes. Examination of a validation set is underway to confirm these preliminary findings.

CITATION INFORMATION: Vallin P, Désy O, Béland S, Wagner E, De Serres S. Monocytic TNF-α Response to EBV Peptides as a Mean to Identify Over-Immunosuppressed Kidney Transplant Recipients. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Vallin P, Désy O, Béland S, Wagner E, Serres SDe. Monocytic TNF-α Response to EBV Peptides as a Mean to Identify Over-Immunosuppressed Kidney Transplant Recipients. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/monocytic-tnf-response-to-ebv-peptides-as-a-mean-to-identify-over-immunosuppressed-kidney-transplant-recipients/. Accessed March 7, 2021.

« Back to 2016 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Penis Transplantation: First U.S. Experience.
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Home
  • Search
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.