Mass-Spectrometry Based Discovery of Tubular Injury Marker in Chronic Kidney Disease

J. Kim¹, D. Han¹, J. Jeong², J. Moon¹, S. Lee³, Y. Kim¹, K. Yoo⁴, D. Kim¹, Y. Kim¹, S. Yang¹

¹Seoul National University Hospital, Seoul, Korea, Republic of, ²Veterans Health Service Medical Center, Seoul, Korea, Republic of, ³Kangwon National University Hospital, Kangwon-do, Korea, Republic of, ⁴Ulsan National University Hospital, Ulsan, Korea, Republic of

Meeting: 2020 American Transplant Congress

Abstract number: B-330

Keywords: Fibrosis, Kidney, Methodology, Mice

Session Information

Session Name: Poster Session B: Biomarker Discovery and Immune Modulation

Session Type: Poster Session

Date: Saturday, May 30, 2020

Session Time: 3:15pm-4:00pm

Presentation Time: 3:30pm-4:00pm

Location: Virtual

*Purpose: Renal tubular injury and interstitial fibrosis are inevitable processes in the progression of chronic kidney disease (CKD). The purpose of this study is to identify common proteins expressed in CKD kidney tissues from animal model and injured primary cultured renal tubuloepithelial cells (TEC).

*Methods: Label-free quantitative proteomic analysis based on liquid chromatography- tandem mass spectrometry was performed on kidney tissue obtained from rat CKD model by 5/6 nephrectomy, and quantitative proteomic analysis based on Tandem mass tag was performed on primary cultured human TECs after hypoxic damage for 24 hours. Then, the proteins identified in common from both types of samples were validated.

*Results: When comparing the proteins expressed before and after hypoxic damage in primary cultured TECs, we identified 1,254 differentially expressed proteins (DEP). And rat kidney tissue harvested 8 weeks after 5/6 nephrectomy showed 2,497 DEPs in comparison with sham-operated kidney tissue. Among those DEPs, only 51 proteins showed a significant increase after chronic damage in both types of samples, and they had significant interactions with other proteins associated with inflammation, apoptosis and fibrosis.

*Conclusions: We have identified specific proteins that commonly increase with chronic injury in primary cultured TECs and kidney tissues. Further study for validating the diagnostic value of these proteins for tubular damage in CKD is needed.

To cite this abstract in AMA style:

Kim J, Han D, Jeong J, Moon J, Lee S, Kim Y, Yoo K, Kim D, Kim Y, Yang S. Mass-Spectrometry Based Discovery of Tubular Injury Marker in Chronic Kidney Disease [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/mass-spectrometry-based-discovery-of-tubular-injury-marker-in-chronic-kidney-disease/. Accessed November 24, 2024.

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