Late Onset Primary Graft Dysfunction after Lung Transplantation Correlates with Transplant Arteriosclerosis in a Humanized Mouse Model
1Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany, 2IFB-Tx, Hannover Medical School, Hannover, Germany
Meeting: 2020 American Transplant Congress
Abstract number: B-299
Keywords: Graft failure, Lung transplantation, Mice, Vascular disease
Session Information
Session Name: Poster Session B: Lung: All Topics
Session Type: Poster Session
Date: Saturday, May 30, 2020
Session Time: 3:15pm-4:00pm
Presentation Time: 3:30pm-4:00pm
Location: Virtual
*Purpose: Primary Graft dysfunction (PGD) is one of the most important causes of early mortality after lung transplantation. We investigated the correlation between the incidence of PGD at 0h and 72h after transplantation and the development of transplant arteriosclerosis.
*Methods: Lung transplant recipients were evaluated for PGD 0h and 72h after transplantation. Surplus donor pericardiacophrenic artery was transplanted into immunodeficient mice. Depending on the group, recipient mice obtained peripheral blood mononuclear cell (PBMC) reconstitution with or without regulatory T-cell (Treg cell) application or were transplanted solely. Luminal occlusion was quantified histologically.
*Results: PBMC enriched grafts which developed PGD 72h after transplantation also showed a significantly higher luminal occlusion in the humanized mouse model (p=0.03) compared to control. Treg cell application prevents luminal occlusion more effectively in arterial xenografts whose lung grafts did not develop PGD either 0h or 72h after transplantation (p=0.14). Luminal stenosis was not significantly different in grafts without PBMC reconstitution regardless whether the respective lung developed PGD or not (p=0.49).
*Conclusions: We could show that an increased PGD after 72h has a significantly more negative effect on the vessel than an increased PGD after 0h. Treg cells are capable to prevent transplant arteriosclerosis in our humanised mouse model.
To cite this abstract in AMA style:
Franz M, Siemeni T, Knöfel A, Sommer W, Avsar M, Ius F, Tudorache I, Kühn C, Falk CS, Warnecke G, Haverich A. Late Onset Primary Graft Dysfunction after Lung Transplantation Correlates with Transplant Arteriosclerosis in a Humanized Mouse Model [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/late-onset-primary-graft-dysfunction-after-lung-transplantation-correlates-with-transplant-arteriosclerosis-in-a-humanized-mouse-model/. Accessed May 16, 2025.« Back to 2020 American Transplant Congress