Ischemia Reperfusion Injury (IRI) Causes Local Release of Free Heme Which Aggravates Inflammation and Contributes to Delayed Graft Function.

F. Gueler,¹ R. Chen,¹ A. Thorenz,¹ K. Madyaningrana,² J. Bräsen,³ H. Haller,¹ S. Immenschuh,³ V. Vijayan.³

¹Nephrology, Hannover Medical School, Hannover, Germany
²Transfusion Medicine, Hannover Medical School, Hannover, Germany
³Pathology, Hannover Medical School, Hannover, Germany

Meeting: 2017 American Transplant Congress

Abstract number: A173

Keywords: Inflammation, Ischemia, Kidney transplantation, Mice

Session Information

Session Name: Poster Session A: Ischemic Injury and Organ Preservation Session I

Session Type: Poster Session

Date: Saturday, April 29, 2017

Session Time: 5:30pm-7:30pm

Presentation Time: 5:30pm-7:30pm

Location: Hall D1

Ischemia reperfusion injury (IRI) is relevant in solid organ transplantation and contributes to delayed graft function (DGF). In this study, release of free heme after renal IRI and the consecutive inflammatory response were studied in mice.

Methods: Renal IRI was induced by 15, 35 and 45 min unilateral renal pedicle clamping in mice. Sham surgery served as control. In a kidney transplant model prolonged cold ischemia time was used to induce IRI of the graft. Mice were sacrificed at 2 and 4 and 24 hours after IRI. Free heme was measured in the kidney and in the circulating blood. qPCR for pro-inflammatory cytokine expression, histology and immunohistochemistry for acute kidney injury were done.

Results: In correlation with duration of ischemia the free heme generation in the tissue increased. Increasing ischemia time resulted in enhanced local pro-inflammatory cytokine release (TNF-alpha, MCP-1, IL-6) and more severe neutrophil infiltration.

Conclusion: Free heme release in ischemic organs aggravates local inflammation. Strategies to reduce free heme production prior to solid organ transplantation.

CITATION INFORMATION: Gueler F, Chen R, Thorenz A, Madyaningrana K, Bräsen J, Haller H, Immenschuh S, Vijayan V. Ischemia Reperfusion Injury (IRI) Causes Local Release of Free Heme Which Aggravates Inflammation and Contributes to Delayed Graft Function. Am J Transplant. 2017;17 (suppl 3).

To cite this abstract in AMA style:

Gueler F, Chen R, Thorenz A, Madyaningrana K, Bräsen J, Haller H, Immenschuh S, Vijayan V. Ischemia Reperfusion Injury (IRI) Causes Local Release of Free Heme Which Aggravates Inflammation and Contributes to Delayed Graft Function. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/ischemia-reperfusion-injury-iri-causes-local-release-of-free-heme-which-aggravates-inflammation-and-contributes-to-delayed-graft-function/. Accessed June 1, 2025.

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