Date: Sunday, April 30, 2017
Session Time: 2:30pm-4:00pm
Presentation Time: 2:30pm-2:42pm
Background: We recently found that angiotensin II type 1 receptor antibody (AT1R-Ab) was associated with decreased renal function in pediatric renal transplant recipients independent of rejection. TNF-α, IL-1β, IL-8, IFN-γ, IL-17, and IL-6 have been associated with vascular inflammation and AT1R activity in hypertension. scleroderma, and pre-eclampsia, but their role in renal transplant patients with AT1R-Ab is unknown. We aimed to assess the relationship between cytokine profiles, biopsy specimens and AT1R-Abs to understand the activity of AT1R mediated inflammatory pathways in these patients.
Methods: 65 pediatric patients were monitored for 2 years post-transplant from August 2005 to November 2014. AT1R-Ab (ELISA test) and TNF-α, IL-1β, IL-8, IFN-γ, IL-17, IL-6 (Luminex assay) were measured and biopsy was obtained at 6 months (m), 12m, 24m[PM1] post-transplant and during episodes of kidney transplant rejection (n=233 samples). A value > 17 units/ml was considered positive for AT1R-Ab. Biopsies were evaluated by Banff 2013 criteria.
Results: In 233 samples, 61% were AT1R-Ab negative and 39% were positive. There were 130 samples with matching biopsy data and 26% had acute rejection and 5% had glomerulitis or arteritis on biopsy. Elevated TNF-α, IL-1β, IL-8, IFN-γ, IL-17, and IL-6 levels were associated with AT1R-Ab positivity but not acute rejection (Table 1). TNF-α, IL-1β, and IL-6 were associated with glomerulitis or arteritis on renal biopsy (Table 1).
Conclusions: Elevated TNF-α, IL-1β, and IL-6 levels are associated with AT1R-Ab positivity and vasculitis, but not rejection. Our data provides evidence that AT1R-Abs activate AT1R mediated inflammatory pathways in renal transplantation that may mediate vascular damage independent of rejection.
CITATION INFORMATION: Pearl M, Grotts J, Rossetti M, Zhang Q, Palma Diaz M, Gjertson D, Weng P, Reed E, Tsai E. Inflammatory Cytokines Are Associated with Angiotensin II Type 1 Receptor Antibodies and Vascular Inflammation in Pediatric Renal Transplantation. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Pearl M, Grotts J, Rossetti M, Zhang Q, Diaz MPalma, Gjertson D, Weng P, Reed E, Tsai E. Inflammatory Cytokines Are Associated with Angiotensin II Type 1 Receptor Antibodies and Vascular Inflammation in Pediatric Renal Transplantation. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/inflammatory-cytokines-are-associated-with-angiotensin-ii-type-1-receptor-antibodies-and-vascular-inflammation-in-pediatric-renal-transplantation/. Accessed November 23, 2020.
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