Impact of Cytokine Gene Polymorphism on Clinical Outcome of Renal Transplantation.
1Nephrologist, Hospital Clinico San Carlos, Madrid, Spain
2UGC Immunology, Hospital Clinico San Carlos and Instituto de Investigación Sanitaria (IdISSC), Madrid, Spain
Meeting: 2017 American Transplant Congress
Abstract number: A183
Keywords: Kidney transplantation, Polymorphism, Rejection
Session Information
Session Name: Poster Session A: Kidney Complications I
Session Type: Poster Session
Date: Saturday, April 29, 2017
Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Hall D1
Cytokine production is subject to genetic regulation, in such a way that gene polymorphisms at the level of promoter or coding regions can alter their levels and thus modify the inflammatory and/or immunologic response towards any stimulus.
Objetive: To evaluate the influence of single nucleotide polymorphisms (SNPs) of IL10, TNFα, IFNγand IL18on early renal graft outcomes.
Method:Observational study that included 709 consecutive patients who received a first renal transplant at our center from January 2005 to December 201. SNP analysis was carried out by real-time PCR using Taqman® probes.Patients were stratified according to the higher production genotype. A control group that included healthy subjects was used to confirm that the genotypic data obtained conformed to the expected frequencies in accordance with the Hardy-Weinberg equilibrium.
Results: There was a significant association between SNP of TNFα -308 G/A and acute vascular rejection (AVR) in the adjusted logistic regression model.Allele A (GA/AA) carriers had nearly a threefold higher risk of developing AVR (OR=2.64; CI 95%: 1.46-4.76; p=0.001) compared to those who had GG genotype.In the analysis for risk of delayed graft function (DGF), stratified according to donor type, an association between DGF and SNP of TNFα -308 G/Ain grafts received from brain dead donors was found. A further association was observed between DGF and SNP of IL18 -137 G/C in grafts received from non-beating heart donors. Regarding renal graft recipients from brain dead donors, TNFα GA/AA genotypes had a higher risk of DGF (OR=6.15; CI 95%: 1.65-22.86; p=0.007);while in those from non-beating heart donors, G allele carriers within SNP -137 G/C of IL18 had the higher risk (OR=2.76; CI 95%: 1.03-7.40; p=0.042).
Conclusions:SNP -308G/A of TNFα can be considered as a non-invasive risk biomarker for AVR and, in grafts coming from brain dead donors, DGF.On the other hand, SNP -137G/C of IL18 was the main genetic marker for DGF in grafts from non-beating heart donors. The knowledge of these polymorphisms previous to transplantation could aid in individualizing immunosuppressant treatment to avoid the development of complications and improve outcomes.
CITATION INFORMATION: Pérez-Flores I, Santiago J, Moreno Dela Higuera A, Calvo N, Rodriguez-Cubillo B, Urcelay E, Shabaka A, Calvo M, Sanchez-Fructuoso A. Impact of Cytokine Gene Polymorphism on Clinical Outcome of Renal Transplantation. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:
Pérez-Flores I, Santiago J, Higuera AMorenoDela, Calvo N, Rodriguez-Cubillo B, Urcelay E, Shabaka A, Calvo M, Sanchez-Fructuoso A. Impact of Cytokine Gene Polymorphism on Clinical Outcome of Renal Transplantation. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/impact-of-cytokine-gene-polymorphism-on-clinical-outcome-of-renal-transplantation/. Accessed October 15, 2024.« Back to 2017 American Transplant Congress