Date: Tuesday, May 2, 2017
Session Name: Poster Session D: Diagnostics/Biomarkers Session II
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Hall D1
Background: Following transplantation, a set of coordinated events occurs within the recipient immune system. Immature dendritic cells (DCs) migrate to sites of inflammation, become activated and process antigen to present to naïve T cells (indirect allorecognition). Recently, we reported that important roles of DCs in kidney transplantation 1). In these experiments, we focused on the cellular interplay involved in the indirect pathway of allorecognition.
Method: We have now adapted the use of intravital microscopy (IVM) to track EYFP DC motility, morphology, and cell density in real-time 2). Fluorescently-labeled syngeneic T cells (CD4: red, CD8: blue) were injected into B6 CD11c-EYFP recipients immediately following transplantation. At various time points, images of the graft, draining lymph nodes and spleen were obtained.
Results: 1. Cellular interaction was detected at graft (Day2 of post txs.). 2. CD4+ T cell had longer interaction with DC compared to CD8+T cell. 3. Three-dimensional analysis confirmed its interaction.
Conclusion: By monitoring the complex behavior of cells, it will possible to answer many of outstanding questions in the field. Targeting “Indirect Pathway” could be a new therapeutic concept in transplantation.
References: 1: Batal I, et al. JASN 2015, 2: Ueno T, et al. Transplant Int. 2016.
CITATION INFORMATION: Ueno T, McGrath M, Yeung M, Jung K, Kihara Y, Konno O, Nakamura Y, Iwamoto H, Yun S, Chandraker A. Imaging Cell Biology in Transplantation. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Ueno T, McGrath M, Yeung M, Jung K, Kihara Y, Konno O, Nakamura Y, Iwamoto H, Yun S, Chandraker A. Imaging Cell Biology in Transplantation. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/imaging-cell-biology-in-transplantation/. Accessed November 23, 2020.
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