Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Hall 4EF
Background: Graft immunocomplex capture fluorescence analysis (ICFA) is an attractive method to detect intra-graft donor-specific anti-HLA antibodies (HLA-DSA). In ABO incompatible (ABOi) transplantation, anti-A/B antibodies are also considered as important DSA (ABO-DSA). Thereupon, it is also useful to monitor intra-graft ABO-DSA to assess AMR by applying the ICFA technique.
Methods: To capture A/B antigens, anti-Band III, VW, and PLVAP beads were composed. The allograft specimen was dissolved in PBS by the lysis buffer. Anti A/B antibodies were added for a positive control sample. Subsequently, A/B antigens were captured by anti-Band III, VW, or PLVAP beads. Then, the immune complexes were detected by anti-human IgG antibodies (PE), analyzed by a Luminex system.
Results: Although Band III and VW beads indicated false-positive/negative, PLVAP beads were capable to capture A/B antigens with high sensitivity and specificity (>95%) provided that a ratio (sample/blank beads MFI) >1.0 was considered as positive. By immunohistological examination, the proximity in A/B antigens and PLVAP expression was confirmed. Report of a case: Consecutive four biopsies following an ABOi renal transplant recipient (A to O)were analyzed. With desensitization by rituximab and DFPP, the post-transplantation course was uneventful. Graft ICFA showed as follows: 3.2 (1h, s-Cr 4.0 mg/dl titer IgG/M:512/8, g0, ptc0, C4d1); 1.8 (4 days, s-Cr 2.3, titer 256/16, g0, ptc0, C4d3); 1.2 (22 days, s-Cr 1.6, titer 128/8, g0, ptc2, C4d3). This result indicated that the remnant ABO-DSA were adsorbed, and subsequently removed from the allograft successfully.
Conclusions: This application would detect intra-graft ABO-DSA, which could lead to a correct diagnosis and shed light on the ABO-DSA kinetics following ABOi transplantation.
CITATION INFORMATION: Nakamura T., Ushigome H., Imanishi Y., Kawai S., Shirouzu T., Osaka M., Masuda K., Matsuyama T., Harada S., Nobori S., Yoshimura N. Graft Immunocomplex Capture Fluorescence Analysis Can Detect Intra-Graft Anti-Blood Group A/B Antibodies Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Nakamura T, Ushigome H, Imanishi Y, Kawai S, Shirouzu T, Osaka M, Masuda K, Matsuyama T, Harada S, Nobori S, Yoshimura N. Graft Immunocomplex Capture Fluorescence Analysis Can Detect Intra-Graft Anti-Blood Group A/B Antibodies [abstract]. https://atcmeetingabstracts.com/abstract/graft-immunocomplex-capture-fluorescence-analysis-can-detect-intra-graft-anti-blood-group-a-b-antibodies/. Accessed April 24, 2019.
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