ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Extended Criteria Donors Have Higher Expression of Inflammatory Genes Than Standard Donors

M. Abbud-Filho,1,2,3 M. Fernandes-Charpiot,1,2 M. Baptista,1,2 H. Caldas,1,2 G. Florim.1

1Medicine, Laboratory of Immunology and Experimental Transplantation - LITEX, Medical School of Sao Jose do Rio Preto, Sao Jose do Rio Preto, Sao Paulo, Brazil
2Transplant, Kidney Transplant Unit, Hospital de Base, FAMERP/FUNFARME, Sao Jose do Rio Preto, Sao Paulo, Brazil
3Urology and Nephrology Institute, Sao Jose do Rio Preto, Sao Paulo, Brazil.

Meeting: 2018 American Transplant Congress

Abstract number: A16

Keywords: Brain death, Donors, marginal

Session Information

Date: Saturday, June 2, 2018

Session Name: Poster Session A: Biomarkers, Immune Monitoring and Outcomes

Session Time: 5:30pm-7:30pm

 Presentation Time: 5:30pm-7:30pm

Location: Hall 4EF

Related Abstracts
  • Effect of Distinct Immunosuppressive Protocols On the Profile of Cytokines Expression in Renal Transplant Recipients of Extended Criteria Donor Kidneys
  • Preimplantation and Post-Transplant Serial Biopsies of Standard and Extended Criteria Donor Kidneys: Intragraft Molecular Profiles and Effect of Different Immunosuppressive Protocols.

Background.Changes to an organ occur at the time of brain death where a series of inflammatory modify occur within the organ. Because it is not known whether this inflammatory environment similarly affects the kidneys from extended (ECD) and standard (SCD) criteria donors. We have previously reported that preimplantation kidney biopsies from ECD donors have a heavier inflammatory profile when compared with SCD donors. In the present study we extended our results using great number of genes and seeking to identify some immunologic pathways involved in the mechanism of sterile inflammation. Methods. Biopsies pretransplant were obtained from ECD (n=40), SCD (n=40) and control groupconsistedof sample with SCDkidney criteria donors without DGF and acute rejection. Gene expression was profile measured by Real Time qPCR Array representing expression levels of genes indicative of inflammation (IL-10, IL-1b, TNF-α, MCP-1, NFK-b, TLR-4, HMGB1, IFN-gamma, TGF-b, Myd-88), cytoprotection (HO-1, HIF-1a), apoptose (CASP-1) and intercellular adhesion (ICAM-1 ) and correlated with donor variables. Results.ECD donors were older had more cerebrovascular accident, arterial hypertension and diabetes (p<0.01). Renal function and 24h proteinuria were worst 1 year after transplantation (p<0.006). Genes IL-10, IL-1 β, TLR-4, HMGB-1, HIF-1 and CASP-1 were significantly more expressed in biopsies from ECD than SCD. ECD kidneys showed significant increase in the transcripts of IL-10, IL-1 β, TNF-α, CCL-2, TLR-4, HMGB-1, TGF-β, HIF-1 than from control, while SCD kidney showed only significantly higher expression of IL-10. Presence of DGF, acute rejection, after one year were not associated with any individual transcript. Conclusions:The present results confirm our previous findings that ECD kidney is highly inflamed when compared with SCD and that a Myd-88 independent pathway of innate immunity be activated.

CITATION INFORMATION: Abbud-Filho M., Fernandes-Charpiot M., Baptista M., Caldas H., Florim G. Extended Criteria Donors Have Higher Expression of Inflammatory Genes Than Standard Donors Am J Transplant. 2017;17 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Abbud-Filho M, Fernandes-Charpiot M, Baptista M, Caldas H, Florim G. Extended Criteria Donors Have Higher Expression of Inflammatory Genes Than Standard Donors [abstract]. https://atcmeetingabstracts.com/abstract/extended-criteria-donors-have-higher-expression-of-inflammatory-genes-than-standard-donors/. Accessed December 5, 2019.

« Back to 2018 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Penis Transplantation: First U.S. Experience.
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Tacrolimus Dose and Trough Blood Levels in a US Liver Transplant Population
  • Penis Transplantation: First U.S. Experience.
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Tacrolimus Dose and Trough Blood Levels in a US Liver Transplant Population
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2019 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

wiley-new-logo

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.