Background.Changes to an organ occur at the time of brain death where a series of inflammatory modify occur within the organ. Because it is not known whether this inflammatory environment similarly affects the kidneys from extended (ECD) and standard (SCD) criteria donors. We have previously reported that preimplantation kidney biopsies from ECD donors have a heavier inflammatory profile when compared with SCD donors. In the present study we extended our results using great number of genes and seeking to identify some immunologic pathways involved in the mechanism of sterile inflammation. Methods. Biopsies pretransplant were obtained from ECD (n=40), SCD (n=40) and control groupconsistedof sample with SCDkidney criteria donors without DGF and acute rejection. Gene expression was profile measured by Real Time qPCR Array representing expression levels of genes indicative of inflammation (IL-10, IL-1b, TNF-α, MCP-1, NFK-b, TLR-4, HMGB1, IFN-gamma, TGF-b, Myd-88), cytoprotection (HO-1, HIF-1a), apoptose (CASP-1) and intercellular adhesion (ICAM-1 ) and correlated with donor variables. Results.ECD donors were older had more cerebrovascular accident, arterial hypertension and diabetes (p<0.01). Renal function and 24h proteinuria were worst 1 year after transplantation (p<0.006). Genes IL-10, IL-1 β, TLR-4, HMGB-1, HIF-1 and CASP-1 were significantly more expressed in biopsies from ECD than SCD. ECD kidneys showed significant increase in the transcripts of IL-10, IL-1 β, TNF-α, CCL-2, TLR-4, HMGB-1, TGF-β, HIF-1 than from control, while SCD kidney showed only significantly higher expression of IL-10. Presence of DGF, acute rejection, after one year were not associated with any individual transcript. Conclusions:The present results confirm our previous findings that ECD kidney is highly inflamed when compared with SCD and that a Myd-88 independent pathway of innate immunity be activated.

CITATION INFORMATION: Abbud-Filho M., Fernandes-Charpiot M., Baptista M., Caldas H., Florim G. Extended Criteria Donors Have Higher Expression of Inflammatory Genes Than Standard Donors Am J Transplant. 2017;17 (suppl 3).

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