Objective To investigate mechanisms of regulation on hepatocyte proliferation by miRNAs. Methods Normal hepatocyte in ivtro was transfected with vectors harboring Ad5/anti-miR26a or Ad5/miR26a, and cell proliferation was tested by MTS assay, cell cycle analysis and apoptosis analysis by flow cytometry, and expressions of p53,cyclin D2 and cyclin E2 in mRNA and protein level, were detected with quantitative real-time PCR and western blot. Results The hepatocyte proliferation was obviously increased (1.47±0.15, p < 0.01), and number of liver cell in G1 phase was more (83.18%±1.70%, p < 0.01) and apoptotic cells was decreased (4.6%±0.96%, p < 0.05) (Figure 1), and expression of cyclin D2 (1.64±0.27, p < 0.01) and cyclin E2 (1.41±0.24, p < 0.05) was enhanced markedly and the expression of p53 (0.51±0.12, p < 0.01) decreased in Ad5/anti-miR26a group compared with control group. In addition, miR26a overexpression showed converse results. Conclusions miR26a is crucial for the regulation of hepatocyte proliferation through the detailed mechanism that directly regulate the cyclin D2 and cyclin E2 and induced p53 mediated apoptosis.

CITATION INFORMATION: Zhou J, Yuan X, Ju W, Jiao X, Wang D, Zhu X, He X. Experimental Study of Hepatocyte Proliferation Regulated by miR26a. Am J Transplant. 2017;17 (suppl 3).

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