Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Exhibit Hall E
Detection of Polyomavirus(Py) DNA in metropolitan river water worldwide has led to the suggestion that primary viral infection can occur by the oral route. The aim of this study was to test this notion experimentally and to compare this route of transmission with the more widely recognized respiratory route.
Mouse Py (MPyV) LID -1 strain was propagated in NIH 3T3 cells and 1×109 genomic equivalents were used to infect 4 week old, 15-20 gram, male, C57BL/6J mice by the nasal or gastric route(n=24 in each group). The Qiagen DNeasy Blood & Tissue Kit was used to extract viral DNA from different organs. Viral load kinetics was studied in groups of 4 mice, sacrificed 3, 7, 10, 14, 21 and 28 days after infection. For quantitation of viral load, MPyV A2 large T antigen gene derived primers were used in a SyBrGreen based PCR run on the ABI Prism 7500 System using a MPyV-PYA3 plasmid based standard curve.
Following nasal infection MPyV DNA was readily detected at virtually all time points with a peak observed on day 10 (see table below).
|Organ||3 days post-infection||7 days post-infection||10 days post-infection||14 days post-infection||21 days post-infection||28 days post-infection|
|Oral||ND||0.5± 2.4||-0.8± 2.5||-1.8±2.4||2.9±0.6||-1.0±2.4|
It is concluded that the oral route of MPy infection is successful, not as efficacious as the respiratory route, and associated with delayed viral dissemination as well as a lower peak MPy load in individual organs.
To cite this abstract in AMA style:Huang G, Zeng G, Huang Y, Randhawa P. Evaluation of the Gastrointestinal Tract as a Potential Route of Primary Polyomavirus Infection [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/evaluation-of-the-gastrointestinal-tract-as-a-potential-route-of-primary-polyomavirus-infection/. Accessed July 19, 2018.
« Back to 2015 American Transplant Congress