ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Evaluation of Function and TCR signaling Profile of CD57+ T Cells in Allo-Specific Immunity.

H. Xu,1 T. Brennan,1 Q. Li,2 A. Kirk.1

1Department of Surgery, Duke University School of Medicine, Durham, NC
2Department of Immunology, Duke University School of Medicine, Durham, NC

Meeting: 2017 American Transplant Congress

Abstract number: B6

Keywords: Co-stimulation, Effector mechanisms, T cell activation, T cell receptors (TcR)

Session Information

Session Name: Poster Session B: Acute and Chronic Rejection

Session Type: Poster Session

Date: Sunday, April 30, 2017

Session Time: 6:00pm-7:00pm

 Presentation Time: 6:00pm-7:00pm

Location: Hall D1

Our studies have showed that CD57+ cells are resistant to B7 costimulation blockade (CoB). We hypothesize that CD57+ cells are highly sensitive to T cell receptor (TCR) stimulation and undertook this study to explore the phenotypic and functional profile of activated CD57+ cells.PBMC from normal subjects (n=12) were assessed by FACS for senescence marker CD57 and the memory subsets of CD57+ cells were defined by CCR7/CD45RA classification. Allo-specific memory response was defined as TNF-a/IFN-g producers following stimulation by mature allogeneic dendritic cells (DC). TCR and intracellular mTOR levels in CD57+ and CD57- cells were quantified using BD QuanTiBRITE. Purified CD57+ and CD57– cells were stimulated with OKT3 or PMA followed by PhosFlow to detect ZAP70, mTOR, and ERK phosphorylation. MicroRNA (miR) profiles were evaluated by RT-PCR. CD57+ cells were largely CCR7–CD45RA– effector memory cells (TEM, CD4+ 70.6±16.5%, CD8+ 61.4±19.3%). Allo-specific CD8+ TNF-a/IFN-g producers (1.56±0.57%) after DC stimulation were mainly CD57+ cells (86.4±14.4%). TCR levels on CD4+CD57+ cells were significantly less (717±166 TCR/cell) than CD4+CD57– cells (947±237 TCR/cell, p=0.016). TCR levels on CD8+CD57+ cells were similar to CD8+CD57– cells. Significantly higher mTOR levels were present in CD8+CD57– cells (7081±1593 mTOR/cell) compared to CD8+CD57+ cells (4821±819 mTOR/cell, p=0.001). CD4+CD57+ and CD4+CD57– cells showed similar mTOR levels. OKT3 induced higher levels of ZAP70 phosphorylation in CD57+ cells than CD57– cells (p<0.05). PMA induced higher phosphorylation for ERK and mTOR in CD57– than CD57+ cells. The miR181 levels were 2 to157 fold higher in CD57+ than CD57– cells (p<0.05). However, the miR17-92 cluster was 3 to 8 fold higher in CD57– than CD57+ cells (p<0.05). The ERK and mTOR phosphorylation in CD57+ cells was similar to CD57– cells after OKT3 stimulation. In summary, allo-specific T cells are largely CD57expressing TEM cells. This subset is highly sensitive to TCR signaling through the ZAP70 pathway despite lower surface TCR expression. Increased expression of miR-181 in the CD57+ subset may increase susceptibility to allo-peptide antigen in the setting of allograft transplantation and underlie their resistance to CoB observed clinically in transplant recipients. Targeting CD57+ cell intracellular signaling pathways may provide protection against allo-specific memory responses.

CITATION INFORMATION: Xu H, Brennan T, Li Q, Kirk A. Evaluation of Function and TCR signaling Profile of CD57+ T Cells in Allo-Specific Immunity. Am J Transplant. 2017;17 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Xu H, Brennan T, Li Q, Kirk A. Evaluation of Function and TCR signaling Profile of CD57+ T Cells in Allo-Specific Immunity. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/evaluation-of-function-and-tcr-signaling-profile-of-cd57-t-cells-in-allo-specific-immunity/. Accessed May 11, 2025.

« Back to 2017 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences