Donor Microbiota and Its Contribution for the Development of Primary Graft Dysfunction Following Human Lung Transplantation
1Surgery, Washington Univ Sch of Med, St. Louis, MO
2Medicine, Washington Univ Sch of Med, St. Louis, MO.
Meeting: 2015 American Transplant Congress
Abstract number: B201
Keywords: Bacterial infection, Donors, Graft failure, Lung transplantation, marginal
Session Information
Session Name: Poster Session B: Lung- All Topics
Session Type: Poster Session
Date: Sunday, May 3, 2015
Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
Background: The microbiome of the donor lung will be imported to the recipient, and its role in allograft immunity remains speculative. The aims of this study were (1) to define the change in microbial communities between donor and recipient lungs, and (2) to correlate this with the development of primary graft dysfunction (PGD) and allograft rejection.
Methods: A retrospective study of 24 LTx was performed between July 2013 and June 2014. Bronchoalveolar lavage (BAL) was performed on the donor before the lungs were harvested, and BAL was performed on the recipients after transplantation for surveillance. Bacterial 16S rDNA extracted from the bronchoalveolar lavage fluid was amplified using barcoded primers, and PCR products were pooled and sequenced by Illumina MiSeq sequencing. Analysis of the V3 region reads was performed using the QIIME pipeline. Reads were clustered into OTUs by QIIME using UCLUST at a threshold of 97% similarity. Representative sequences from each OTU were aligned by pyNAST to the RDP core reference alignment and a phylogenetic tree was built using fast tree. Alpha and beta diversity were done in QIIME. Multivariate statistical analysis was performed using Primer 6 software.
Results: The bacterial community between donor and transplanted lungs from recipients was taxonomically diversified. The inter-individual variation was greater than intra-individual variation of the microbial community for all LTx recipients. Bacteria in the phyla Actinobacteria (P<0.0001) (class Actinobacteria) has a greater predominance in the recipient lungs over time compared to that in donor lungs. However, the microbiome Firmicutes (P<0.0001) (class Bacilli), and Proteobacteria (P=0.0066) (class Alpha proteobacteria and Gamma proteobacteria) reduced over time after LTx compared those in donor lungs. We performed the association analysis of PGD development with 434 most abundant OTUs and found that there was a greater predominance of Acinetobacter in donor lungs of PGD patients compared to that in non-PGD patients (P=0.025).
Conclusion: The pulmonary microbiome between donor and transplanted lungs from recipients is taxonomically diverse and dynamic over time. The predominance of Acinetobacter in donor lungs was associated with PGD development.
To cite this abstract in AMA style:
Xu Z, Schuessler R, Steward N, Hachem R, Trulock E, Patterson A, Kreisel D, Mohanakumar T. Donor Microbiota and Its Contribution for the Development of Primary Graft Dysfunction Following Human Lung Transplantation [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/donor-microbiota-and-its-contribution-for-the-development-of-primary-graft-dysfunction-following-human-lung-transplantation/. Accessed May 9, 2025.« Back to 2015 American Transplant Congress