ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Direct Intrahepatic Delivery of Pegylated-Catalase Is Protective in a Rat Ischemia-Reperfusion Injury Model.

E. Beal,1 S. Black,1 J. Kim,1 V. Velazquez,2 K. Washburn,1 D. Hayes Jr.,3 B. Whitson.1

1Department of Surgery, Division of Transplant, The Ohio State University Wexner Medical Center, Columbus, OH
2Center for Vaccines and Immunity, The Research Institute at Nationwide Childrens Hospital, Columbus, OH
3Departments of Pediatrics and Internal Medicine, The Ohio State University College of Medicine, Columbus, OH

Meeting: 2017 American Transplant Congress

Abstract number: A159

Keywords: Ischemia, Liver transplantation, Warm ischemia

Session Information

Session Name: Poster Session A: Ischemic Injury and Organ Preservation Session I

Session Type: Poster Session

Date: Saturday, April 29, 2017

Session Time: 5:30pm-7:30pm

 Presentation Time: 5:30pm-7:30pm

Location: Hall D1

Introduction: Ischemia-reperfusion injury (IRI) is characterized by cessation of blood flow to an organ or tissue leading to accumulation of toxic metabolites and free radicals, known as reactive oxygen species. Catalase is an endogenous enzyme involved in H2O2 detoxification. We hypothesize that the delivery of polyethylene glycol-catalase (PEG-CAT), directly targeted to the liver during ischemia would significantly reduce IRI.

Methods and Materials: To characterize the mechanism of protection, endothelial cells and hepatocytes were treated with PEG-CAT using a H2O2 based model of oxidative stress. A 70% rat liver hilar clamp model with catheter based segmental intrahepatic delivery was used to validate the ability of PEG-CAT to mitigate IRI at the organ level.

Results:. Endothelial cells and hepatocytes treated with PEG-CAT demonstrated significantly increased catalase activity, reduced cell toxicity, decreased LDH, and decreased apoptosis. Mitochondrial membrane potential was maintained in endothelial cells treated with H2O2 and PEG-CAT, as demonstrated by JC-1 staining. The intrahepatic segmental hilar clamp model of IRI demonstrated significantly decreased serum ALT (Figure 1), AST and MDA, with improved maintenance of ATP and glutathione in livers treated with PEG-CAT.

Conclusions: In cells, treatment with PEG-CAT is capable of decreasing H2O2 cytotoxicity, with maintenance of cellular metabolism. Catheter based intrahepatic delivery of PEG-CAT mitigates hepatic IRI. Potential applications of PEG-CAT using this methodology exist for hepatic surgery and liver transplantation. PEG-CAT could be used to pre-treat a donor organ either immediately after explantation via an ex-vivo perfusion circuit prior to transplantation to treat existing injury and to prevent reperfusion injury.

CITATION INFORMATION: Beal E, Black S, Kim J, Velazquez V, Washburn K, Hayes Jr. D, Whitson B. Direct Intrahepatic Delivery of Pegylated-Catalase Is Protective in a Rat Ischemia-Reperfusion Injury Model. Am J Transplant. 2017;17 (suppl 3).

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

To cite this abstract in AMA style:

Beal E, Black S, Kim J, Velazquez V, Washburn K, Jr DHayes, Whitson B. Direct Intrahepatic Delivery of Pegylated-Catalase Is Protective in a Rat Ischemia-Reperfusion Injury Model. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/direct-intrahepatic-delivery-of-pegylated-catalase-is-protective-in-a-rat-ischemia-reperfusion-injury-model/. Accessed May 28, 2025.

« Back to 2017 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences