ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Diagnostic Value and Confounding Factors of Urinary Biomarkers in the Non-Invasive Screening of Renal Allograft Inflammation

A. Vermorel,1 A. Devresse,1 C. Tinel,1,2 V. Sauvaget,2 L. Morin,2 L. Amrouche,1,2 M. Rabant,2,3 D. Anglicheau.1,2

1Renal Transplantation Unit, Necker-Enfants Malades Hospital, Paris, France
2INSERM U1173, Institut Necker Enfants Malades, Paris, France
3Pathology, Necker-Enfants Malades Hospital, Paris, France.

Meeting: 2018 American Transplant Congress

Abstract number: 462

Keywords: Kidney transplantation, Polyma virus, Rejection, Urinalysis

Session Information

Date: Tuesday, June 5, 2018

Session Name: Concurrent Session: Kidney: Polyoma

Session Time: 2:30pm-4:00pm

 Presentation Time: 3:06pm-3:18pm

Location: Room 2AB

Related Abstracts
  • Multicenter Valdiation of Urinary Biomarkers in Kidney Transplantation: Urinary CXCL9 Protein Correlates with Acute Rejection and Deterioration of Kidney Function
  • Urinary CXCL10 Independently Improves the Noninvasive Diagnosis of Antibody-Mediated Kidney Allograft Rejection

Introduction

The value of urinary biomarkers has already been demonstrated in diagnosing acute rejection of the renal allograft, but confounding factors, which may interfere with their interpretation, have been poorly evaluated. Our goal was to evaluate the respective impact of urinary tract infection, reactivation of BK virus (BKV) and acute rejection on these biomarkers in order to optimize a noninvasive strategy of acute rejection assessment.

Methods

A total of 391 urine samples collected in 330 patients at time of graft biopsy and BKV blood nucleic acid testing were included. Urinary concentrations of the CXCL9 and CXCL10 proteins and of CD3ε, CXCL9, CXCL10, granzyme B and perforin mRNAs were quantified. Serum DSA status at the time of biopsy was also determined by luminex® single antigen.

Results

Our results confirmed the impact of urinary tract infection on the urinary chemokine protein levels (1,6±2,1 vs 0,7±1,8; p<0.005 for CXCL9 and 2,1±1,7 vs 0,8±2,0; p<0,001 for CXCL10). During reactivation of BKV, while viruria had minimal impact, concentrations of urinary RNA (mainly CXCL9, CXCL10 and granzyme B) and protein biomarkers were significantly increased and remained similar in viremic patients and patients with proven BKV nephritis. After elimination of these confounding factors and multivariate analysis, a 3-parameter diagnostic model (urinary CXCL9 and CXCL10 proteins and serum DSA status) best diagnosed acute rejection with an area under the curve of 0.81 (P=9.93E-13). Restricted to unstable patients at time of indication biopsy (n=168), the rejection prediction was even more robust with an AUC of 0.85 (P = 5.09E-12).

Conclusion

After exclusion of confounding factors such as urinary tract infection and BK virus viremia, a model associating CXCL9 and CXCL10 protein levels and DSA status can predict acute rejection with a high accuracy, especially in unstable patients. Moreover, BKV viremia and BKV nephritis have similar urinary inflammatory signature.

CITATION INFORMATION: Vermorel A., Devresse A., Tinel C., Sauvaget V., Morin L., Amrouche L., Rabant M., Anglicheau D. Diagnostic Value and Confounding Factors of Urinary Biomarkers in the Non-Invasive Screening of Renal Allograft Inflammation Am J Transplant. 2017;17 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Vermorel A, Devresse A, Tinel C, Sauvaget V, Morin L, Amrouche L, Rabant M, Anglicheau D. Diagnostic Value and Confounding Factors of Urinary Biomarkers in the Non-Invasive Screening of Renal Allograft Inflammation [abstract]. https://atcmeetingabstracts.com/abstract/diagnostic-value-and-confounding-factors-of-urinary-biomarkers-in-the-non-invasive-screening-of-renal-allograft-inflammation/. Accessed December 15, 2019.

« Back to 2018 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Penis Transplantation: First U.S. Experience.
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Tacrolimus Dose and Trough Blood Levels in a US Liver Transplant Population
  • Penis Transplantation: First U.S. Experience.
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Tacrolimus Dose and Trough Blood Levels in a US Liver Transplant Population
  • How To Handle a Missed or Delayed Dose Intake? A Pharmacokinetic Study of Cyclosporine and Tacrolimus in Renal Transplant Patients
  • Hyperkalemia in the Early Post Renal Transplant Period

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2019 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

wiley-new-logo

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.