ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Comparison Between IgG, C1q and C3d Single Antigen Bead Assays in Detecting Class I Complement-Binding Anti-HLA Antibodies.

M. Moreno Gonzales,1 D. Mitema,2 B. Smith,3 C. Schinstock,3 M. Stegall,3 M. Gandhi.3

1Servicio de Cirugía General, Clínica Anglo Americana, Lima, Peru
2Division of Nephrology, Mississippi Baptist Medical Center, Jackson, MS
3Transplant Center, Mayo Clinic, Rochester, MN

Meeting: 2017 American Transplant Congress

Abstract number: A24

Keywords: HLA antibodies

Session Information

Date: Saturday, April 29, 2017

Session Name: Poster Session A: Antibody Mediated Rejection in Kidney Transplant Recipients I

Session Time: 5:30pm-7:30pm

 Presentation Time: 5:30pm-7:30pm

Location: Hall D1

Related Abstracts
  • Clinical Significance of C3d Binding Donor Specific Anti-HLA Antibodies (DSA) Detected by Single Antigen Beads (SAB) After Kidney Transplantation.
  • Utilizing HLA Single Antigen Flow Beads Assays to Evaluate Anti-MAMU Antibody in Sensitized Nonhuman Primate Model.

Introduction. Donor specific antibodies (DSA) are associated with an increased risk of chronic antibody mediated rejection (CAMR). DSA characterization by single antigen bead (SAB) assays has enhanced donor selection. Importantly, complement-binding DSA has a strong association with CAMR. The novel complement-fixing SAB binding assays (C1q/C3d) may add more information to the “traditional” IgG SAB assay (measures total IgG) as far as prediction of CAMR, however data regarding this is lacking. The aim of this study was to compare the sensitivities (s) of the IgG, C1q and C3d SAB assays in detecting complement-binding antibodies.

Methods. Sera (n=20) from sensitized patients, previously used for serologic Class I HLA typing by complement-dependent cytotoxicity (CDC) method were tested with 3 different SAB assays (IgG[undiluted and 1:8 dilution], C1q, C3d). Serological anti-HLA specificities were compared with those obtained by IgG, C1q and C3d SAB assays and sensitivities were established.

Results. 24 complement-fixing specificities were defined by CDC. IgG SAB identified 24 specificities (s 24/24 = 1, OR Infinity), C1q recognized 21 (s 21/24 = 0.875, OR 89.63) and 12 were obtained by C3d assay (s 12/24 = 0.500, OR 48.23).Pearson correlation showed the association between: 1) C1q and IgG SAB assays (cut off C1q SAB 1000 MFI, cut off IgG SAB 5000 MFI: r=0.347 p<0.0001; and 2) C3d and IgG SAB assays (cut off 500 MFI C3d SAB, 5000 MFI for IgG SAB: r= -0.173, p=0.279). Conclusions. For Class I anti-HLA antibodies, IgG SAB was more sensitive in detecting complement-binding antibodies when compared to C1q/C3d SAB assays.

CITATION INFORMATION: Moreno Gonzales M, Mitema D, Smith B, Schinstock C, Stegall M, Gandhi M. Comparison Between IgG, C1q and C3d Single Antigen Bead Assays in Detecting Class I Complement-Binding Anti-HLA Antibodies. Am J Transplant. 2017;17 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Gonzales MMoreno, Mitema D, Smith B, Schinstock C, Stegall M, Gandhi M. Comparison Between IgG, C1q and C3d Single Antigen Bead Assays in Detecting Class I Complement-Binding Anti-HLA Antibodies. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/comparison-between-igg-c1q-and-c3d-single-antigen-bead-assays-in-detecting-class-i-complement-binding-anti-hla-antibodies/. Accessed January 16, 2021.

« Back to 2017 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • How To Handle a Missed or Delayed Dose Intake? A Pharmacokinetic Study of Cyclosporine and Tacrolimus in Renal Transplant Patients
  • How To Handle a Missed or Delayed Dose Intake? A Pharmacokinetic Study of Cyclosporine and Tacrolimus in Renal Transplant Patients
  • Hyperkalemia in the Early Post Renal Transplant Period
  • Direct Oral Anticoagulation Use in Kidney Transplant Recipients

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.