ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Circulating Exosomes Isolated from Human Lung Transplant Recipients with Respiratory Viral Infections Contain Nucleic Acids Specific to Virus and Can Lead to Activation of Stress Proteins

S. Bansal, R. Bremner, M. Smith, T. Mohanakumar

Norton Thoracic Institute, St. Joseph’s Hospital and Medical Center, Phoenix, AZ

Meeting: 2019 American Transplant Congress

Abstract number: 388

Keywords: Infection, Lung transplantation

Session Information

Date: Monday, June 3, 2019

Session Name: Concurrent Session: Infections, Antibodies and Biomarkers in Lung Transplantation

Session Time: 4:30pm-6:00pm

 Presentation Time: 4:54pm-5:06pm

Location: Room 206

Related Abstracts
  • Respiratory Viral Infections Following Human Lung Transplant Can Induce Exosomes with Lung Self-Antigens, Kα1Tubulin and Collagen V.
  • Respiratory Viral Infections Induces Exosomes and Antibodies to Lung Self-Antigens, Kα1Tubulin and Collagen V, Following Human Lung Transplantation

*Purpose: Exosomes are membrane vesicles released by cells into body fluids. Our laboratory demonstrated the presence of circulating exosomes with lung self-antigens (SAgs), Collagen V and K-α Tubulin, and donor HLA in lung transplant recipients (LTxR) undergoing rejection. Since respiratory viral infections (RVI) is a known risk factor for development of chronic rejection following LTx, the goal of this study is to determine whether RVI leads to induction of circulating exosomes with SAgs and to demonstrate the presence of viral DNA/RNA in exosomes isolated from LTxR with RVI.

*Methods: Exosomes were isolated using ultracentrifugation and purity was confirmed by sucrose cushion gradient. DNA and RNA were isolated using kits and quantified on the Nanodrop. Libraries were generated using Kapa Biosystem’s library kit. The raw Illumina 2x150bp pair-end reads were checked on FastQC and were aligned to the human and viral genome build from CHIPseeker Database. Validation was done using Abs and primers to respiratory syncytial virus, coronavirus, and rhinovirus. To determine the role of exosomes in inducing stress and DNA damage, we incubated airway epithelial cell line, KCC266, with exosomes from LTxR with RVI or stable.

*Results: Viral nucleic acid sequences were demonstrable in exosomes from LTxR with RVI. Comparing the sequences with human genome, we identified the presence of DNA sequences specific to defensins and GTPase pathways, 195 unique in LTxR with RVI specific to apoptotic cleavage, NMDA receptor activation and stable had 91 sequences specific to MAP kinase and cell death signaling. We also identified H. influenzae sequences in exosomes isolated from LTxR with influenza. Further, we demonstrated increases in proteins associated with endoplasmic reticulum stress, i.e, PERK, ATF4 and BiP in cells incubated with exosomes isolated from LTxR with RVI. However, significant increase in the levels of STING and IRF3 at the protein levels were not detected.

*Conclusions: Based on these preliminary results we conclude that RVI LTxR leads to induction of circulating exosomes having unique nucleic acid sequences of viral etiology suggesting that these nucleic acids of viral origin may have functional consequences including upregulation of stress markers.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Bansal S, Bremner R, Smith M, Mohanakumar T. Circulating Exosomes Isolated from Human Lung Transplant Recipients with Respiratory Viral Infections Contain Nucleic Acids Specific to Virus and Can Lead to Activation of Stress Proteins [abstract]. Am J Transplant. 2019; 19 (suppl 3). https://atcmeetingabstracts.com/abstract/circulating-exosomes-isolated-from-human-lung-transplant-recipients-with-respiratory-viral-infections-contain-nucleic-acids-specific-to-virus-and-can-lead-to-activation-of-stress-proteins/. Accessed March 8, 2021.

« Back to 2019 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Home
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Search
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Home
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.