Characterization of a Novel CD8+ B Cell Subset in Monkeys Tolerant of Heart Allografts
1Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Boston, MA, 2Transplantation Research Center, Renal Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 3Center for Transplantation Sciences, Division of Cardiac Surgery, Department of Surgery, Massachusetts General Hospital, Boston, MA
Meeting: 2020 American Transplant Congress
Abstract number: D-324
Keywords: B cells
Session Information
Session Name: Poster Session D: B-cell / Antibody /Autoimmunity
Session Type: Poster Session
Date: Saturday, May 30, 2020
Session Time: 3:15pm-4:00pm
Presentation Time: 3:30pm-4:00pm
Location: Virtual
*Purpose: We have previously reported that transient mixed hematopoietic chimerism induced via donor bone marrow transplantation can achieve tolerance to cardiac allografts in non-human primates (NHPs). The purpose of this study was to measure the frequency and properties of a unique subset of CD8+ B cells, recently identified in our laboratory, in tolerant monkeys.
*Methods: Cynomolgus Macaques underwent organ transplantation followed by injection of donor bone marrow cells with mixed chimerism conditioning consisting of 3Gy TBI, 7Gy thymic irradiation, anti-thymocyte globulin, and a short course of anti-CD154 mAb and CyA therapy. The frequencies of different B cell subsets were measured in the peripheral blood of ten monkeys pre- and post-transplantation. In addition, PBMCs were isolated from peripheral blood of two tolerant animals (recipients of heart plus kidney or heart plus thymus cotransplants) 274 and 847 days post-bone marrow and FACS-sorted into five groups: T cells (CD3+), whole B cells (CD20+), mature B cells (CD20+CD8–CD27+), naïve B cells (CD20+CD8–CD27–), and CD8+ B cells (CD20+CD8+). mRNA was extracted from lysates of each cell population and sequenced using an Illumina NextSeq 500.
*Results: During leukocyte reconstitution following the conditioning procedure, CD8+ B cells exhibited a phenotype reminiscent of transitional B cells (CD20+CD27–IgMhiCD38hi) and the percentage of these cells among peripheral B cells was much higher than that observed in naïve animals (23.4% vs 2.5% respectively; p<0.001). In the two tolerant animals, the CD8+ B cells gene expression suggested an immature profile which included the expression of CD21, CD38, and CD24. Interestingly, however, CD8+ B cells showed an increased expression of the gene encoding for the regulatory protein CD72. In addition, CD8+ B cells had greater expression of CD69 which is involved with TGFbeta production.
*Conclusions: The prevalence of CD8+ B cells expressing genes involved in regulatory immunity after bone marrow transplantation suggests that these cells may play a role in tolerance induction to heart allografts in NHPs. Work is in progress to further characterize these novel cells.
To cite this abstract in AMA style:
Morrissette JA, Pruner K, Lee S, Paster J, Sommer W, O J, Hanekamp I, Sage P, Benichou G, Madsen JC. Characterization of a Novel CD8+ B Cell Subset in Monkeys Tolerant of Heart Allografts [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/characterization-of-a-novel-cd8-b-cell-subset-in-monkeys-tolerant-of-heart-allografts/. Accessed May 10, 2025.« Back to 2020 American Transplant Congress