ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Characterisation of Kidney-Specific Extracellular Vesicles During Isolated Organ Normothermic Machine Perfusion

J. Ashcroft1, T. L. Williams2, T. R. Elliott1, A. Marton1, S. A. Hosgood1, M. L. Nicholson1, V. Kosmoliaptsis1

1Department of Surgery, University of Cambridge, Cambridge, United Kingdom, 2Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

Meeting: 2022 American Transplant Congress

Abstract number: 1299

Keywords: Epitopes, FACS analysis, Machine preservation, Perfusion

Topic: Basic Science » Basic Science » 16 - Biomarkers: -omics and Systems Biology

Session Information

Session Name: Biomarkers: -omics and Systems Biology

Session Type: Poster Abstract

Date: Monday, June 6, 2022

Session Time: 7:00pm-8:00pm

 Presentation Time: 7:00pm-8:00pm

Location: Hynes Halls C & D

*Purpose: Introduction: Normothermic machine perfusion (NMP) can evaluate and recondition deceased-donor kidneys but accurate biomarkers are needed to assess organ quality and to predict post-transplant organ function. Extracellular vesicles (EVs) are membrane-delimited microparticles released by all cells and represent ideal targets for biomarker development as they reflect the conditional state of tissues and organs.

*Methods: A donation after brainstem death (DBD) kidney declined for transplantation was subjected to NMP at 36 °C for two hours and perfusate and urine were sampled at 30 minute intervals. EVs were separated using size exclusion chromatography and were characterised by nanoparticle tracking, western-blot and gold-immunolabelled transmission electron microscopy (TEM). Multiparametric flow cytometric (FC) analysis of characteristic surface epitopes and non-labelled mass spectrometry (LC-MS/MS) to identify enriched EV protein markers and associated molecular pathways was performed.

*Results: Separated EVs had mean concentration of 4.36×108-6.61×109 particles/mL and mean size of 130-145nm. Western-blot confirmed the presence of EV associated proteins (CD81, CD63) and TEM showed microparticles consistent with exosomes. FC revealed EV surface upregulation of MHC class II (HLA-DR/DP/DQ), lymphocyte (CD24, CD44), endothelial cell proliferation (CD105), and integrin (CD29) markers during NMP (Figure 1). Proteomic analysis identified 563 proteins in kidney EV cargo, including 75% of the top EV proteins in Vesiclepedia. EV origin was demonstrated through detection of kidney derived markers MME, NHE-RF1/RF3, PODXL, SLC12A1/NKCC2, and AQP1. Gene Ontology analysis associated cellular components to vesicle membranes and KEGG database analysis revealed vesicular proteins were involved in G-protein-coupled receptor signalling and cation homeostasis. Proteomic analysis confirmed EV MHC upregulation during NMP.

*Conclusions: Kidney NMP facilitates isolation and characterisation of tissue-specific EVs which is essential for EV-derived biomarker development in kidney transplantation. EV subpopulation and cargo analyses during NMP may enable assessment of the conditional state of donor organs and provide mechanistic insights into cellular pathways for therapeutic manipulation.

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

To cite this abstract in AMA style:

Ashcroft J, Williams TL, Elliott TR, Marton A, Hosgood SA, Nicholson ML, Kosmoliaptsis V. Characterisation of Kidney-Specific Extracellular Vesicles During Isolated Organ Normothermic Machine Perfusion [abstract]. Am J Transplant. 2022; 22 (suppl 3). https://atcmeetingabstracts.com/abstract/characterisation-of-kidney-specific-extracellular-vesicles-during-isolated-organ-normothermic-machine-perfusion/. Accessed May 30, 2025.

« Back to 2022 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences