Date: Saturday, June 1, 2019
Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Hall C & D
*Purpose: To verify a subset of intragraft and circulating natural killer cells associated with kidney allograft rejection and fibrosis.
*Methods: Thirty-nine clinically indicated transplant biopsies performed at our center from May 2015 to July 2017 were included in this analysis. Paraffin-embedded sections of 39 renal biopsies were labeled using Opal™ multiplex kit (PerkinElmer®). We used anti-CD3, anti-CD56, anti-CD57, anti-CD49b, anti-NKG2A, and anti-KIR antibodies to define NK cells. NK cell was defined if a lymphocyte is CD3-negative, CD56-positive, and at least one of the other surface markers (CD57, CD49b, NKG2A, and KIR)-positive. In addition, we performed peripheral blood mononuclear cell (PBMC) analysis in those patients to determine NK cell subsets.
*Results: According to histopathologic reports, five patients (12.8%) had no major abnormality (NOMOA); 11 (28.2%) had T cell-mediated rejection (TCMR) only; 11 (28.2%) had pathologic features compatible with antibody-mediated rejection (ABMR) only; 9 (23.1%) had both TCMR and ABMR; and 3 (7.7%) had BKVN. Opal™ multiplex labeling revealed that the density of NK cells was very low in NOMOA group (0.17 ± 0.28 /mm2), TCMR only group (0.24 ± 0.35/mm2), and BKVN (0.05 ± 0.08/mm2). On the other hand, the density of NK cells was significantly increased in ABMR only group (1.33 ± 2.43/mm2) and both TCMR and ABMR group (1.1 ± 1.59/mm2). CD56+CD57+ infiltrate was most frequently observed subset of NK cells in every pathologic group. Kaplan-Meier survival analysis revealed that death-censored graft failure was significantly higher in recipients with infiltrating NK cells compared to those without infiltrating NK cells (Log-rank test, p=0.025). In PBMC analysis, CD49b–CD56dim NK cells were significantly decreased in the ABMR only group than the other groups, whereas CD57+NKG2C+KIR–CD56bright NK cells were significantly increased (p<0.05).
*Conclusions: In kidney transplant recipients with ABMR, CD56+CD57+ NK cell is the main subset of NK cell not only in biopsy tissue but also in circulation. Presence of infiltrating NK cells is significantly associated with death-censored graft failure.
To cite this abstract in AMA style:Jung H, Wee Y, Kim J, Choi M, Choi J, Kwon H, Jung J, Kim S, Cho Y, Park Y, Go H, Han M, Kim Y, Han D, Shin S. CD56+CD57+ Infiltrate is a Predominant Subset of Infiltrating Natural Killer Cell in Renal Transplant Biopsies with Antibody-Mediated Rejection [abstract]. Am J Transplant. 2019; 19 (suppl 3). https://atcmeetingabstracts.com/abstract/cd56cd57-infiltrate-is-a-predominant-subset-of-infiltrating-natural-killer-cell-in-renal-transplant-biopsies-with-antibody-mediated-rejection/. Accessed February 27, 2021.
« Back to 2019 American Transplant Congress