Date: Monday, May 1, 2017
Session Name: Concurrent Session: Regulatory Cells in Alloimmunity
Session Time: 4:30pm-6:00pm
Presentation Time: 4:30pm-4:42pm
Background; Previously reports showed that CCR7+CD8+ T cells have suppressive effect on various pathogenic immune cells. The aim of this study is to investigate the regulatory function of CCR7+CD8+ T cells on allo-reactive effector T cells involved in acute rejection in kidney transplantation.
Methods; We investigated the suppressive effect of CCR7+CD8+T cells on T cell proliferation and also inflammatory cytokine induced injury on human primary renal tubular epithelial cells (HPRTEpiC) by FACs analysis and ELISA. Also, we compared the proportion of CCR7+CD8+ T cells in peripheral blood mononuclear cells by FACs analysis between biopsy proven acute rejection (AR)(n=22) and biopsy proven normal histology (NC)(n=16).
Results ;CCR7+CD8+ T cells significantly decreased the proportion of IFN-g, IL-17/CD4+T cells, and production of IL-17 and IL-2 (P<0.05 for each). In contrast, CCR7+CD8+ T cells increased the production of IL-10/CD4+T cells compared to Th17 condition. Second, CCR7+CD8+ T cells significantly reduced IL-6, IL-8 and CCL20 secretion from HPRTEpiC, which have been induced by inflammatory cytokines or activated T cells. Also, Our results showed that CCR7+CD8+ T cells significantly reduced IL-17, IFN-g and IL-2 production from T cells co-cultured with HPRTEpiC.Third, ex vivo analysis of PBMCs isolated from kidney transplant recipients (KTRs) with or without acute rejection, the percentage of CCR7+CD8+ T cells showed significant decrease in the AR group compared to NC group (P<0.05). In contrast, the percentage of CD28nullCD57+ T cell and effector memory CD8 T cell (CD8+TEMRA)increased in the AR group compared to NC group.In peripheral blood from total patients groups, CCR7+CD8+ T cells was inversely correlated with the CD28nullCD57+ T cell and effector memory CD8 T cell (CD8+TEMRA).
Conclusion; This study suggests that the CCR7+CD8+T cells effectively regulate effector T cells associated with allograft rejection, which suggests that use of CCR7+CD8+T cells could be proposed as therapeutic strategy to improve allograft outcome.
CITATION INFORMATION: Chung B, Kim K, Kim B.-M, Doh K, Yang C. CCR7+CD8+ T Cell Suppresses Effector T Cell Related Allo-Immune Responses in Kidney Transplant Recipients. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Chung B, Kim K, Kim B-M, Doh K, Yang C. CCR7+CD8+ T Cell Suppresses Effector T Cell Related Allo-Immune Responses in Kidney Transplant Recipients. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/ccr7cd8-t-cell-suppresses-effector-t-cell-related-allo-immune-responses-in-kidney-transplant-recipients/. Accessed October 24, 2020.
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