Date: Sunday, April 30, 2017
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Hall D1
Background: In HLA-sensitized kidney transplant patients (Pts) the concomitant presence of ABMR/CMR on Bx is associated with a more severe and (&) usually irreversible injury profile compared to either ABMR or CMR alone. Previously, we showed overexpression of antibody-dependent cell-mediated cytotoxicity- & other inflammation-associated genes in Bx of ABMR Pts compared to CMR and no rejection Control. Here we investigated the mRNA transcript profiles for a similar group of genes in Bx from Pts with/without ABMR, CMR or combined ABMR/CMR.
Methods: Total RNA was extracted from 54 Bx (12 ABMR, 10 combined ABMR/CMR, 19 CMR, 13 no rejection Control) for reverse transcription, pre-amplification and qPCR on 25 possibly rejection-associated genes. The results of each gene were presented as relative quantity (RQ) vs. a reference RNA after normalized by housekeeping GAPDH gene. Comparisons were made among 4 groups.
Results: Comparing ABMR, CMR & Control, the mRNA transcript levels of 14 tested genes were significantly elevated in ABMR &/or CMR above Control (results of 6 representative genes shown in Table below). Meanwhile any gene with significant elevation in either ABMR or CMR was also significantly elevated in Pts with combined ABMR/CMR.
*p<0.05 & **0.05≤p<0.1 vs. Control; #p<0.05 & ##0.05≤p<0.1 vs. ABMR; [spades]p<0.05 & [spades][spades]0.05≤p<0.1 vs. CMR
Conclusions: Bx from Pts with combined ABMR/CMR exhibited elevation of gene expression from both ABMR & CMR, suggesting that the immune activations specific to either ABMR or CMR are likely activated simultaneously in combined ABMR/CMR, resulting in a more severe and irreversible injury profile.
CITATION INFORMATION: Zhang H, Nast C, Doldron J, Cheung M, Mirocha J, Kahwaji J, Vo A, Jordan S, Toyoda M. Biopsies (Bx) of Renal Allografts with Antibody- + Cell-Mediated Rejection (ABMR/CMR) Exhibit Gene Expression Signatures from Both Types of Rejection. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Zhang H, Nast C, Doldron J, Cheung M, Mirocha J, Kahwaji J, Vo A, Jordan S, Toyoda M. Biopsies (Bx) of Renal Allografts with Antibody- + Cell-Mediated Rejection (ABMR/CMR) Exhibit Gene Expression Signatures from Both Types of Rejection. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/biopsies-bx-of-renal-allografts-with-antibody-cell-mediated-rejection-abmrcmr-exhibit-gene-expression-signatures-from-both-types-of-rejection/. Accessed June 20, 2021.
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