Introduction:

A novel rodent model of ischaemia- reperfusion injury (IRI) has shown significant improvement in renal function and histology after administration of adipose derived regenerative cells (ADRCs). However the exact mechanism on how these regenerative cells ameliorate IRI is not fully understood. Our research aims to identify the biodistribution – spacial and temporal post therapeutic administration of ADRCs.

Methods:

ADRCs were extracted from the inguinal fat of the Fisher 344 rat, labelled with a near infrared lipophilic dye, DiR, and injected via the renal artery of our surgical IRI rat model. Whole body imaging was performed using the IVIS spectrum imaging system. Organs (kidneys, heart, lung, brain, spleen, liver) were then removed and imaged at various time points and also imaged post ClearT2 tissue clearance.

Results:

Alone, DiR labelled cells strongly fluoresced. Whole body scanning of the rat post ADRC injection at various timepoints demonstrated no signal from DiR-labelled ADRCs. However, high ADRC cell numbers (>2×10^5) were detected in various ex vivo organs at early time points. Low cell numbers (<2×10^5) were undetectable. Clearing the ex vivo organs with ClearT2 did not improve visibility in select tissues.

Discussion:

Histology and cytometry will be performed to refine localization of injected ADRCs. Data will support our experiments that investigate microenviromental effects of ADRCs.

CITATION INFORMATION: Ghita R, Lathan R, Hillyard D, Mark P, Clancy M. Biodistribution of Adipose Derived Regenerative Cells Administered via the Renal Artery in Novel Rat Transplant Model. Am J Transplant. 2017;17 (suppl 3).

« Back to 2017 American Transplant Congress