Association of Genetic Polymorphisms of Angiopoietin-Like 4 with Proteinuria in Kidney Allograft Recipients.
1Mendez National Institute of Transplantation Foundation, Los Angeles, CA
2St. Vincent Medical Center, Los Angeles, CA
3Taipei medical university, Taipei, Taiwan
4Western University of Health Sciences, Pomona.
Meeting: 2016 American Transplant Congress
Abstract number: D263
Keywords: Gene polymorphism, Kidney transplantation, Proteinuria
Session Information
Date: Tuesday, June 14, 2016
Session Name: Poster Session D: Poster Session II: Kidney Complications-Other
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Halls C&D
Background: Proteinuria is a hallmark of glomerular injury, and persistent proteinuria is associated with graft failure in kidney transplant patients. Recently, it is known that the level of circulating angiopoietin-like 4 (ANGPTL4), which is secreted from skeletal muscle, heart, and adipose tissues, is elevated in the patients with human nephrotic syndrome. In this model, ANGPTL4 is responsible for reducing proteinuria with accompanied rise in free triglycerides.
Purpose: The purpose of this study is to determine effect of genetic polymorphism of ANGPTL4 on proteinuria after kidney transplantation.
Methods: 282 patients out of 400 renal transplant patients between 2008 and 2012 at St. Vincent Medical Center were studied in a retrospective study design. The level of proteinuria was measured by random urine protein to creatinine ratio (UPC ratio), and divided into three groups (Normal/Moderate: UPC ≤ 500 mg, Severe: > 500 mg and ≤ 3500 mg, Nephrotic: > 3500 mg). Single nucleotide polymorphisms of ANGPTL4 gene (rs1044250, rs2278236, rs116843064, rs11672433, rs4076317) were determined by real time PCR with sequence specific primers.
Results: Statistical differences were found in genetic polymorphism of ANGPTL4 (rs1044250, rs2278236) in regards to proteinuria among tested patients. rs1044250 (C/T missense mutation) alleles showed multiple significant differences between Normal/Moderate proteinuria group and other groups (Normal/Moderate vs. Severe: CC vs. CT+TT: OR=0.549, CI=0.334-0.903, p=0.01766, Normal/Moderate vs. Severe+Nephrotic: CC vs CT+TT: OR=0.489, CI=0.304-0.787, p=0.00309). Similar trends were found in rs2278236 (A/G) alleles with statistical significances (Normal/Moderate vs. Severe: GG vs. AG+AA: OR=0.609, CI=0.359-1.032, p=0.06446, Normal/Moderate vs. Severe+Nephrotic: GG vs. AG+AA: OR=0.527, CI=0.319-0.873, p=0.01225). Other SNPs did not show statistically significant differences between different proteinuria groups.
Conclusions: This study suggests that the presence of T allele of rs1044250 and A allele of rs2278236 with ANGPTL4 is associated with lower risk of severe to nephrotic range of proteinuria in renal transplant patients.
CITATION INFORMATION: Chang Y, Shah T, Chuang Y.-T, Min D. Association of Genetic Polymorphisms of Angiopoietin-Like 4 with Proteinuria in Kidney Allograft Recipients. Am J Transplant. 2016;16 (suppl 3).
To cite this abstract in AMA style:
Chang Y, Shah T, Chuang Y-T, Min D. Association of Genetic Polymorphisms of Angiopoietin-Like 4 with Proteinuria in Kidney Allograft Recipients. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/association-of-genetic-polymorphisms-of-angiopoietin-like-4-with-proteinuria-in-kidney-allograft-recipients/. Accessed March 8, 2021.« Back to 2016 American Transplant Congress