Assessment of the Usefulness of Urine Signature to Differentiate Acute Rejection in a Prospective Kidney Transplantation Cohort
1Department of Core Research Laboratory, Medical Science Institute, Kyung Hee University Hospital at Gangdong, Seoul, Korea, Republic of, 2Department of Internal Medicine, Division of Nephrology, Kyung Hee University Hospital at Gangdong, Seoul, Korea, Republic of, 3Department of Internal Medicine, Division of Nephrology, College of Medicine, Kyung Hee University, Seoul, Korea, Republic of, 4Department of Internal Medicine, Division of Nephrology, Kyung-pook National University School of Medicine, Daegu, Korea, Republic of, 5Department of Internal Medicine, Division of Nephrology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea, Republic of, 6Department of Internal Medicine, Sungkyunkwan University, Samsung Hospital, College of Medicine, Seoul, Korea, Republic of, 7Department of Internal Medicine, Division of Nephrology, Busan Paik Hospital, College of Medicine, Inje University, Busan, Korea, Republic of
Meeting: 2020 American Transplant Congress
Abstract number: B-321
Keywords: Gene expression, Kidney transplantation, Non-invasive diagnosis, Rejection
Session Information
Session Name: Poster Session B: Biomarkers, Immune Assessment and Clinical Outcomes
Session Type: Poster Session
Date: Saturday, May 30, 2020
Session Time: 3:15pm-4:00pm
Presentation Time: 3:30pm-4:00pm
Location: Virtual
*Purpose: Despite many biomarker studies in the field of transplantation, development and validation of noninvasive molecular assays to diagnose disease and monitor immune activity is seriously needed. Previously, we developed and validated the urinary mRNA signature to predict and monitor immune status from cross-sectional ARTKT-1 study.
*Methods: We assessed the usefulness of the urine specific gene set to differentiate rejection in a prospective kidney transplant recipient cohort (ARTKT-2) study, which composed of 415 newly transplanted patients from October 2015. Urine samples were collected at 2 weeks and 3, 6, 12, 18 and 24 months after kidney transplantation. To test AR signature composed of 6-gene set in urine, the gene expression was measured in 90 patients who experienced the rejection events or had no episode of clinical event during post-OP 1 year.
*Results: We measured the expression levels of six genes in urine and used AR algorithm developed by binary logistic regression analysis of gene expression data. Expression of urine gene set were significantly higher in patients with acute rejection as compared with stable graft function. The AR predicted probability using urine signature was significantly increased in patients with acute rejection. In particular, the AR predicted probability was highest at 2-weeks after kidney transplantation in all patients, but the probability gradually decreased over time after transplantation. We also found that urine signature gradually increased from 1-3 months before the diagnosis of pathologic diagnosis.
*Conclusions: We validated the expression of AR specific gene signature in our prospective cohort samples and suggest our urine rejection signature could be an indicator of immune activation and predict future rejection.
To cite this abstract in AMA style:
Seo J, Jung S, Moon J, Jeong K, Kim C, Chung B, Park J, Kim Y, Lee S. Assessment of the Usefulness of Urine Signature to Differentiate Acute Rejection in a Prospective Kidney Transplantation Cohort [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/assessment-of-the-usefulness-of-urine-signature-to-differentiate-acute-rejection-in-a-prospective-kidney-transplantation-cohort/. Accessed May 16, 2025.« Back to 2020 American Transplant Congress