Objective: Renal ischemia-reperfusion injury (IRI) is very important issue in kidney transplantation. Nitric oxide (NO) was well known to be a protector of IRI. The aim of this study was to investigate the gene expression of the protective effect of the new delivery system of the NO releasing nanofiber on renal IRI in a rat models. Methods: Male Sprague-Dawley rats were divided into three groups: (1) sham group (SG, n=5); (2) control group, renal IRI without any treatment (CG, n=4); (3) NO group, the renal IRI with wrapping the liver using NO rapid releasing-polymer nanofiber matrix (NG, n=6). After Rt nephrectomy, NO releasing sheet was applied by wrapping Lt kidney one hour before clamp of renal artery. Renal ischemia was sustained during 55 minutes, followed by reperfusion. NO sheet was removed 24 hours. And 48 hours after surgery, the rats were sacrificed. We investigated the changes of gene expression between the groups with RNA Quant-Seq analysis. Results: Mean of creatinine in SG, CG and NG at 48 hours after operation were 0.48(±0.08), 4.67(±0.33) and 2.60(±1.0) respectively. There were significant differences between groups. (p=0.002) The top five terms of upregulated gene ontology (GO) among this study that the gene content of CG is more than two times than that of SG, and the gene content of NG is less than two times than that of CG were GO:0046777~protein autophosphorylation, GO:0006468~protein phosphorylation, GO:0042981~regulation of apoptotic process, GO:0045893~positive regulation of transcription, and GO:0018105~peptidyl-serine phosphorylation. The top five terms of downregulated GO were GO:0052697~xenobiotic glucuronidation, GO:0008152~metabolic process, GO:0052696~flavonoid glucuronidation, GO:0009813~flavonoid biosynthetic process, and GO:0055114~oxidation-reduction process. Conclusion: NO nanofiber has the protective effect against rat renal IRI. This genetic bioinformation may present the understanding the mechanism of protective effect of NO.

CITATION INFORMATION: Ahn H, Joo S, Ko H, Jung K, Shin J. A Study on Gene Expression of the Protective Effect of Nitric Oxide Releasing Nanofiber in Rat Renal Ischemia-Reperfusion Injury. Am J Transplant. 2017;17 (suppl 3).

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