Experimental Porcine Pancreatic Allotransplantation after Hypothermic Perfusion

T. Prudhomme¹, D. Kervella¹, K. Renaudin², V. Gauttier¹, S. Le Bas-Bernardet³, D. Minault³, J. Hervouet³, D. Cantarovich¹, G. Karam¹, G. Blancho¹, J. Branchereau¹

¹Institut de Transplantation Urologie Néphrologie (ITUN), CHU Nantes, Nantes, France, ²Département d’Anatomie et de Cytologie Pathologique, CHU Nantes, Nantes, France, ³Centre de Recherche en Transplantation et Immunologie, UMR1064, INSERM, Université de Nantes, Nantes, France

Meeting: 2020 American Transplant Congress

Abstract number: B-339

Keywords: Ischemia, Pancreas transplantation, Pig

Session Information

Session Name: Poster Session B: Ischemia Reperfusion & Organ Rehabilitation

Session Type: Poster Session

Date: Saturday, May 30, 2020

Session Time: 3:15pm-4:00pm

Presentation Time: 3:30pm-4:00pm

Location: Virtual

*Purpose: The main objective of this study was to compare the different preservation techniques (static cold storage (SCS) and hypothermic pulsatile perfusion (HPP)) in a model of porcine pancreatic allotransplantation on insulin-dependent diabetic pigs.

*Methods: We compared static cold storage (SCS) (n=3) and hypothermic pulsatile perfusion (HPP) (n=3) of pancreatic transplants and set up an experimental model of pancreatic allotransplantation after SCS (n=4) or HPP (n=4), on insulin-dependent diabetic pigs.

*Results: The levels of pancreatic suffering markers during preservation were higher in the SCS group. On histological analysis, after 6 hours of preservation, the pancreatic histological lesions were similar in the HPP and SCS groups. After 12h and 24h of preservation, pancreatic histological lesions were more important in the HPP group. We performed 14 pancreatic allotransplantations on insulin-dependent diabetic pigs: 6 after SCS (AlloTx-SCS 2h ; n= 3 and AlloTx-SCS 6h ; n= 3) and 8 after HPP (AlloTx-HPP 2h ; n= 4 and AlloTx-HPP 6h ; n= 4). A total of 8 immediate post-operative deaths occurred, related to surgical or anesthetic complications. No vascular thrombosis occurred. 1 animal in the AlloTx-SCS 2h group and 1 animal in the AlloTx-HPP 2h group were not diabetic after transplantation and the 2 animals in the AlloTx-HPP 6h group were not diabetic after transplantation.

*Conclusions: To our knowledge, we report the first experimental pancreatic transplants on a diabetic pig model after preservation by hypothermic pulsatile perfusion, without early transplant thrombosis. Hypothermic pulsatile infusion improves transplant preservation conditions when it is less than 12 hours.

To cite this abstract in AMA style:

Prudhomme T, Kervella D, Renaudin K, Gauttier V, Bas-Bernardet SLe, Minault D, Hervouet J, Cantarovich D, Karam G, Blancho G, Branchereau J. Experimental Porcine Pancreatic Allotransplantation after Hypothermic Perfusion [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/experimental-porcine-pancreatic-allotransplantation-after-hypothermic-perfusion/. Accessed November 21, 2024.

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