Correlation Between Level of Biomarkers Measured in Perfusate of Machine-Preserved ECD/DCD Kidneys and Post-Transplant Graft Function
1Cardiff Transplant Unit, University Hospital for Wales, Cardiff, United Kingdom
2The Medical Biochemistry and Immunology Department, University Hospital for Wales, Cardiff, United Kingdom.
Meeting: 2015 American Transplant Congress
Abstract number: D69
Keywords: Donors, Kidney transplantation, Machine preservation, non-heart-beating, Preservation
Session Information
Session Name: Poster Session D: Immunology, Biomarkers and Immunosuppression
Session Type: Poster Session
Date: Tuesday, May 5, 2015
Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
Background:
DCD and ECD kidneys are associated with higher risk of failure compare to SCD kidneys. Therefore we see increasing need to identify sensitive tool to assess quality of these kidneys prior transplantation and predict their outcomes.
Hypothermic machine perfusion (HMP) is known to improve transplant outcomes compare to static storage but cannot be used as sensitive kidney quality assessment tool. However, it was suggested that monitoring of biomarkers in HMP perfusate could correlate with early post-transplant outcomes.
Some novel biomarkers (e.g. NGAL, KIM-1, AST, LDH) appears offering better accuracy compare to conventional biomarkers (i.e. creatinine) for the diagnosis of Acute Kidney Damage. We conducted study to determine correlation between concentration of NGAL, KIM-1, AST, LDH in machine perfusate of ECD/DCD kidneys and transplant outcomes.
Study:
Total, we studied 10 kidney grafts preserved on a LifePort®, perfused with KPS-1 solution. Perfusate was sampled at 15 minutes, 1 hour, 2 and 3 hours. We measured level of NGAL, KIM-1, AST and LDH using automated ELISA assay and correlate with donor/recipient demographics and kidney outcomes (DGF, PNF, eGFR at 1, 3 and 6 months).
Results:
Concentration of all biomarkers steadily increased during first 3 hours of HMP. Level of each biomarker at every time point well correlated with kidney function (eGFR) at 1, 3 and 6 months post-transplantation. Eight kidneys developed functional DGF but we found no correlation with the level of biomarkers in this group. No one kidney developed PNF.
Conclusion:
Our data indicate that concentration of all biomarkers in perfusate correlates with post-transplant kidney function. Determination of those four biomarkers potentially can be used as sensitive kidney quality assessment tool but more data are required.
To cite this abstract in AMA style:
Ablorsu E, Zouwail S, Laftsidis P, Cook B, Szabo L. Correlation Between Level of Biomarkers Measured in Perfusate of Machine-Preserved ECD/DCD Kidneys and Post-Transplant Graft Function [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/correlation-between-level-of-biomarkers-measured-in-perfusate-of-machine-preserved-ecddcd-kidneys-and-post-transplant-graft-function/. Accessed May 19, 2025.« Back to 2015 American Transplant Congress