Date: Monday, May 4, 2015
Session Time: 4:00pm-5:30pm
Presentation Time: 5:12pm-5:24pm
Location: Room 119-A
Pre-transplant B cell depletion in NHP with Rituximab (IgG1 anti-CD20 mAb) combined with cyclosporin A has been shown to promote the survival of islets allografts. At the same time, B cell deficient uMT mice were shown to lack a donor-specific memory T cell response after transplantation. This suggests that B cell depletion could be used to prevent the development of T cell memory response in transplantation. Our group has studied the effect of anti-CD20 mediated B cell depletion on the memory T cell alloresponse in naïve and allosensitized mice. Wild-type and anti-OVA TCR transgenic mice were treated with an IgG2a anti-CD20 monoclonal antibody (5D2, Genentech), 5 days prior to skin transplantation, which depleted nearly all B cells in the peripheral blood and secondary lymphoid organs, but only reduced the B cell frequency in the bone marrow. Even though no effect was appreciated on the memory T cell direct alloresponse, the indirect response was significantly increased after skin transplantation of naïve mice. Furthermore, in allosensitized mice, anti-CD20 mAb treatment enhanced both direct and indirect reactivation of allospecific memory T cells and accelerated second set rejection of skin allografts. This suggests that the effect of anti-CD20 antibodies on alloimmunity and allograft rejection might vary upon the nature of the antibodies as well as the circumstances under which they are delivered.
To cite this abstract in AMA style:Marino J, Crosby P, Paster J, Trowell A, Briggs K, Maxwell L, Benichou G. B Cell Depletion With an Anti-CD20 Antibody Enhances Alloreactive Memory T Cell Response After Transplantation [abstract]. Am J Transplant. 2015; 15 (suppl 3). http://atcmeetingabstracts.com/abstract/b-cell-depletion-with-an-anti-cd20-antibody-enhances-alloreactive-memory-t-cell-response-after-transplantation/. Accessed December 17, 2017.
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