Articles tagged "Warm ischemia"

2017 American Transplant Congress
Objective Assessment of Graft Metabolism and Function During Ex Vivo Liver Perfusion.
J. Echeverri,¹ S. Cleland,² M. Kaths,¹ I. Linares,¹ R. Roizales,¹ N. Goldaracena,¹ D. Kollmann,¹ M. Hamar,¹ P. Urbanellis,¹ S. Ganesh,¹ O. Adeyi,³ D. Grant,¹ N. Selzner,⁴ M. Wasowicz,² M. Selzner.¹
¹Multi-Organ Transplant Program, Department of Surgery, University of Toronto - Toronto General Hospital, Toronto, ON, Canada; ²Multi-Organ Transplant Program, Department of Anesthesia, University of Toronto - Toronto General Hospital, Toronto, ON, Canada; ³Multi-Organ Transplant Program, Department of Pathology, University of Toronto - Toronto General Hospital, Toronto, ON, Canada; ⁴Multi-Organ Transplant Program, Department of Medicine, University of Toronto - Toronto General Hospital, Toronto, ON, Canada
During EVLP no objective perfusion parameters have been found to predict outcome after transplantation.Methods: Livers with 4grades of injury were perfused for 4 hours and…
2017 American Transplant Congress
Ex-Situ Extended Limb Preservation: A Protocol for Porcine Limb Perfusion.
E. Dalla Pozza,¹ E. Duraes,¹ M. Madajka,¹ R. Frautschi,¹ K. Cho,¹ N. Dawlagala,¹ V. Fahradyan,¹ H. Shah,¹ C. Cakmakoglu,¹ C. Quintini,² F. Papay,¹ A. Rampazzo,¹ B. Bassiri Gharb.¹
¹Plastic Surgery, Cleveland Clinic, Cleveland, OH; ²General Surgery, Cleveland Clinic, Cleveland, OH
Background: Ischemia and reperfusion injury remains a significant limiting factor for the success of vascularized composite allotransplantation. Compared to the cold storage of the procured…
2017 American Transplant Congress
Improved Preservation During Normothermic Ex-Vivo Liver Perfusion with Delta Opioid Agonist (D-Ala2, D-Leu5) Enkephalin (DADLE).
E. Beal,¹ J. Kim,¹ D. Hayes Jr.,² K. Washburn,¹ B. Whitson,¹ S. Black.¹
¹Department of Surgery, Division of Transplant, The Ohio State University Wexner Medical Center, Columbus, OH; ²Departments of Pediatrics and Internal Medicine, The Ohio State University Wexner Medical Center, Columbus, OH
Background: The metabolic demand of donor livers during prolonged normothermic ex-vivo perfusion with its associated oxygen stress is a challenge. Delta opioid agonist (D-Ala2, D-Leu5)…
2016 American Transplant Congress
Protective Effects of Nrf2 Over-Activation in Myeloid Cells During Hepatic Ischemia Reperfusion Correlates with Stat3 Signaling in Hepatocytes.
C. Harberg,¹ L.-Y. Lee,¹ S. Werner,² D. Johnson,³ J. Johnson,³ D. Foley.¹
¹Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI; ²Biology, Institute of Molecular Health Sciences, Swiss Federal Institute of Technology, Zurich, Switzerland; ³Pharmaceutical Sciences, University of Wisconsin School of Pharmacy, Madison, WI.
Ischemia-reperfusion injury (IRI) is a consequence of liver resection and liver transplantation. The Nrf2-antioxidant response element pathway is a key mediator of cellular defense against…
2016 American Transplant Congress
Reciprocal Effects of HDAC1 and HDAC2 Deletion on Renal Cold Ischemia Survival and Nuclear Co-Repressor Complex Expression.
D. Aufhauser Jr,¹ D. Murken,¹ Z. Wang,¹ A. Samanta,² G. Ge,¹ T. Bhatti,³ W. Hancock,^2,3 M. Levine.¹
¹Surgery, University of Pennsylvania, Philadelphia, PA; ²Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA; ³Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA.
Introduction: Class I histone/protein deacetylases (HDACs) 1 and 2 bear >85% protein sequence homology and conventionally are thought to have similar function via occupancy in…
2016 American Transplant Congress
Short, Cool and Well Oxygenated – HOPE for DCD Kidneys.
P. Kron, A. Schlegel, O. de Rougemont, C. Oberkofler, P.-A. Clavien, P. Dutkowski.
University Hospital, Zurich, Switzerland.
Background Organs from donors after cardiac death (DCD) suffer from severe loss of function requiring development of innovative methods of preservation. A novel perfusion approach,…
2016 American Transplant Congress
Hydrogen Sulfide Supplementation Helps Mitigate Effects of Ischemia Reperfusion Injury in a Murine Model of Donation After Cardiac Death Renal Transplantation.
J. Grewal,¹ I. Lobb,¹ M. Saha,² J. Jiang,² A. Haig,¹ A. Sener.^1,2
¹Microbiology & Immunology, University of Western Ontario, London, Canada; ²Matthew Mailing Centre for Translational Transplant Studies, London Health Sciences Centre, London, Canada.
Donation after cardiac death(DCD) renal grafts experience a combination of prolonged warm and cold ischemia leading to greater ischemia reperfusion injury(IRI) that results in higher…
2016 American Transplant Congress
Predictors of Urologic Complications After Pediatric En-Bloc Donor Kidney Transplantation.
S. Kabagambe,¹ A. De Mattos,² R. Perez,¹ C. Santhanakrishnan,¹ J. Sageshima,¹ C. Troppmann,¹ J. McVicar.¹
¹Surgery, University of California-Davis, Sacramento, CA; ²Transplant Nephrology, University of California-Davis, Sacramento, CA.
Pediatric en-bloc kidney transplant (PEBKT) has expanded the donor pool. Association of donor characteristics and urologic complications is not well described. The objective of this…
2016 American Transplant Congress
Resveratrol Attenuates Liver IRI by Regulating Macrophage Activation Through SIRT1/p19/p53 Pathway.
K. Nakamura,¹ M. Zhang,² B. Ke,¹ S. Kageyama,¹ R. Busuttil,¹ J. Araujo,² J. Kupiec-Weglinski.¹
¹Surgery, Liver Transplant, University of California-Los Angeles, Los Angeles, CA; ²Medicine, Division of Cardiology, University of California-Los Angeles, Los Angeles, CA.
Background/Aim: Sirtuin1 (SIRT1), a NAD+-dependent type III histone/protein deacetylase, plays a key role in inflammation responses and stress resistance. Although SIRT1 may regulate innate activation…
2016 American Transplant Congress
Differential Effect of Delayed Graft Function (DGF) on Kidney Transplant Outcomes: When Does DGF Not Make a Difference?
M. Khan,¹ H. El-Hennawy,¹ A. Farney,¹ J. Rogers,¹ G. Orlando,¹ A. Reeves-Daniel,² A. Palanisamy,² M. Gautreaux,³ S. Iskandar,³ W. Doares,⁴ S. Kaczmorski,⁴ R. Stratta.¹
¹Surgery, Wake Forest, Winston-Salem, NC; ²Internal Medicine, Wake Forest, Winston-Salem, NC; ³Pathology, Wake Forest, Winston-Salem, NC; ⁴Pharmacy, Wake Forest, Winston-Salem, NC.
Delayed graft function (DGF), defined as need for dialysis in 1st week following kidney transplant (KT), is an early surrogate marker of organ quality and…